Sauro Salvatore, Di Renzo Simona, Castagnola Raffaella, Grande Nicola M, Plotino Gianluca, Foschi Federico, Mannocci Francesco
Biomaterials, Biomimetics & Biophotonics, King's College London Dental Institute at Guy's, King's College and St. Thomas' Hospitals, Floor 17, Guy's Hospital, London, UK.
Am J Dent. 2011 Feb;24(1):25-30.
To evaluate the bond strength of resin dentin interfaces created with adhesives applied on root dentin using the water wet or ethanol wet bonding technique. The morphology of resin dentin interfaces was evaluated using confocal microscopy.
Four experimental resin adhesives (R#A to R#D) and one commercial three-step/etch and rinse adhesive were applied to the root canal dentin of endodontically treated single canal incisors using the water (control) or ethanol wet bonding technique. The ethanol wet bonding substrate was achieved by keeping the root canal immersed in absolute ethanol (100%) for 3 minutes. The root dentin bonded specimens were sectioned into beams, stored in distilled water (24 hours) and finally tested for microtensile bond strengths (tTBS). Additional dentin surfaces were conditioned and bonded as previously described. They were prepared for the microscopy study and finally observed using confocal microscopy.
The ethanol wet bonding technique gave higher bond strength values for all the adhesives tested: in Group 1 (water wet bonding technique) no significant difference was found between the resins tested; the only exception being the most hydrophilic Resin #4 showing the highest bond strength values (P < 0.05). In Group 2 (ethanol wet bonding technique) no statistical differences were present between Resin #A and Resin #D. Resin #C showed the highest bond strength values. Confocal microscopy showed better resin diffusion and hybrid layer formation when the ethanol wet bonding was used.
评估使用水湿或乙醇湿粘结技术将粘结剂应用于牙根牙本质所形成的树脂-牙本质界面的粘结强度。使用共聚焦显微镜评估树脂-牙本质界面的形态。
使用水（对照）或乙醇湿粘结技术，将四种实验性树脂粘结剂（R#A至R#D）和一种市售三步/酸蚀冲洗粘结剂应用于经根管治疗的单根管切牙的根管牙本质。通过将根管浸入无水乙醇（100%）中3分钟来实现乙醇湿粘结底物。将粘结有牙根牙本质的标本切成小梁，保存在蒸馏水中（24小时），最后测试微拉伸粘结强度（tTBS）。按照先前描述的方法对其他牙本质表面进行处理和粘结。将它们制备用于显微镜研究，最后使用共聚焦显微镜进行观察。
对于所有测试的粘结剂，乙醇湿粘结技术给出了更高的粘结强度值：在第1组（水湿粘结技术）中，测试的树脂之间未发现显著差异；唯一的例外是最亲水的树脂#4显示出最高的粘结强度值（P < 0.05）。在第2组（乙醇湿粘结技术）中树脂#A和树脂#D之间不存在统计学差异。树脂#C显示出最高的粘结强度值。共聚焦显微镜显示，当使用乙醇湿粘结时，树脂扩散和混合层形成更好。
