Dainezi Vanessa Benetello, Iwamoto Alexsandra Shizue, Martin Airton Abrahão, Soares Luís Eduardo Silva, Hosoya Yumiko, Pascon Fernanda Miori, Puppin-Rontani Regina Maria
Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba, Departamento de Odontopediatria, Piracicaba, SP, Brasil.
Universidade Federal do Piauí, Campus Ministro Petrônio Portella, Departamento de Física , CCN Bairro Ininga, Teresina, PI, Brasil.
J Appl Oral Sci. 2017 Jan-Feb;25(1):101-111. doi: 10.1590/1678-77572016-0053.
This study evaluated the effect of different filling pastes and cleaning agents on the root dentin of primary teeth using Fourier-transformed Raman spectroscopy (FT-Raman), micro energy-dispersive X-ray fluorescence (µ-EDXRF) and scanning electron microscopic (SEM) analysis.
Eighty roots of primary teeth were endodontically prepared and distributed into 4 groups and filled according to the following filling pastes: Control-no filling (CP), Calen®+zinc oxide (CZ), Calcipex II® (CII), Vitapex® (V). After seven days, filling paste groups were distributed to 4 subgroups according to cleaning agents (n=5): Control-no cleaning (C), Ethanol (E), Tergenform® (T), 35% Phosphoric acid (PA). Then, the roots were sectioned and the dentin root sections were internally evaluated by FT-Raman, µ-EDXRF and SEM. Data was submitted to two-way ANOVA and Tukey tests (α=0.05).
Regarding filling pastes, there was no significant difference in organic content. CP provided the lowest calcium values and, calcium/phosphoric ratio (Ca/P), and the highest phosphoric values. For cleaning agents there was no difference in organic content when compared to the C; however, T showed significantly higher calcium and Ca/P than PA. All groups showed similar results for phosphorus. The dentin smear layer was present after use of the cleaning agents, except PA.
The filling pastes changed the inorganic content, however they did not change the organic content. Cleaning agents did not alter the inorganic and organic content. PA cleaned and opened dentin tubules.
本研究采用傅里叶变换拉曼光谱（FT - Raman）、微能量色散X射线荧光光谱（µ - EDXRF）和扫描电子显微镜（SEM）分析，评估不同充填糊剂和清洗剂对乳牙牙根牙本质的影响。
选取80颗乳牙牙根进行根管预备，分为4组，并根据以下充填糊剂进行充填：对照组 - 不充填（CP）、Calen® + 氧化锌（CZ）、Calcipex II®（CII）、Vitapex®（V）。7天后，根据清洗剂将充填糊剂组再分为4个亚组（n = 5）：对照组 - 不清洗（C）、乙醇（E）、Tergenform®（T）、35%磷酸（PA）。然后，将牙根切片，通过FT - Raman、µ - EDXRF和SEM对牙本质根切片进行内部评估。数据采用双向方差分析和Tukey检验（α = 0.05）。
关于充填糊剂，有机成分无显著差异。CP组的钙含量、钙/磷比（Ca/P）最低，磷含量最高。对于清洗剂，与C组相比，有机成分无差异；然而，T组的钙和Ca/P显著高于PA组。所有组的磷含量结果相似。使用清洗剂后，除PA组外，均存在牙本质玷污层。
充填糊剂改变了无机成分，但未改变有机成分。清洗剂未改变无机和有机成分。PA清洗并开放了牙本质小管。
