School of Agriculture, Meijo University, Tenpaku-ku, Nagoya, Aichi, Japan.
J Agric Food Chem. 2011 May 25;59(10):5222-30. doi: 10.1021/jf2004095. Epub 2011 Apr 15.
A rapid bacterial identification method by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) using ribosomal proteins coded in S10 and spc operons as biomarkers, named the S10-GERMS (the S10-spc-alpha operon gene encoded ribosomal protein mass spectrum) method, was applied for the genus Bacillus a Gram-positive bacterium. The S10-GERMS method could successfully distinguish the difference between B. subtilis subsp. subtilis NBRC 13719(T) and B. subtilis subsp. spizizenii NBRC 101239(T) because of the mass difference of 2 ribosomal subunit proteins, despite the difference of only 2 bases in the 16S rRNA gene between them. The 8 selected reliable and reproducible ribosomal subunit proteins without disturbance of S/N level on MALDI-TOF MS analysis, S10, S14, S19, L18, L22, L24, L29, and L30, coded in S10 and spc operons were significantly useful biomarkers for rapid bacterial classification at species and strain levels by the S10-GERMS method of genus Bacillus strains without purification of ribosomal proteins.
一种基于核糖体蛋白 S10 和 spc 操纵子编码的 S10-GERMS（S10-spc-α操纵子基因编码核糖体蛋白质谱）方法的基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）快速细菌鉴定方法，已应用于革兰氏阳性菌芽孢杆菌属。由于 2 种核糖体亚基蛋白的质量差异，S10-GERMS 方法能够成功区分枯草芽孢杆菌亚种枯草亚种 NBRC 13719(T)和枯草芽孢杆菌亚种 spizizenii NBRC 101239(T)之间的差异，尽管它们的 16S rRNA 基因仅相差 2 个碱基。在 MALDI-TOF MS 分析中，S/N 水平不受干扰的 8 种可靠且可重复的核糖体亚基蛋白 S10、S14、S19、L18、L22、L24、L29 和 L30，这些蛋白编码在 S10 和 spc 操纵子中，对于快速细菌分类非常有用，可用于芽孢杆菌属菌株的种和株水平的快速细菌分类，而无需核糖体蛋白的纯化。
