Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Physiol Res. 2011;60(1):185-8. doi: 10.33549/physiolres.932131.
M(1)-muscarinic acetylcholine (ACh) receptors (M(1)R) were directly demonstrated immunocytochemically in electronmicroscopic images of rat diaphragm neuromuscular junctions (NMJ). Specific electron-dense granules were located at presynaptic nerve ending membranes and in the sarcolemma in the depths of postsynaptic folds. This first visualization of M(1)R on both sides of the NMJ is in agreement with previous pharmacological data on the regulatory role of M(1)R in quantal and non-quantal ACh release.
M(1)-毒蕈碱型乙酰胆碱 (ACh) 受体 (M(1)R) 在大鼠膈肌神经肌肉接头 (NMJ) 的电子显微镜图像中直接通过免疫细胞化学显示。特异性电子致密颗粒位于突触前神经末梢膜和突触后褶皱深处的肌膜中。这是首次在 NMJ 的两侧直接观察到 M(1)R，与之前关于 M(1)R 在量子和非量子 ACh 释放中的调节作用的药理学数据一致。
