鉴定新型单核细胞增生李斯特氏菌 PCR 血清群 profile IVb-v1。
Characterization of the novel Listeria monocytogenes PCR serogrouping profile IVb-v1.
机构信息
Institut Pasteur, WHO Collaborating Centre and French National Reference Centre for Listeria, Microbes and Host Barriers Group, Paris, France.
出版信息
Int J Food Microbiol. 2011 May 14;147(1):74-7. doi: 10.1016/j.ijfoodmicro.2011.03.010. Epub 2011 Mar 21.
The World Health Organization Collaborating Centre for Listeria (WHOCCL) has developed in 2004 a multiplex PCR assay that separates the 4 major Listeria monocytogenes serovars (1/2a, 1/2b, 1/2c, and 4b) into distinct PCR serogroups. A new PCR profile has been recently identified, constituted of amplified DNA fragments of prs, ORF2819, ORF2110 and lmo0737. Here we characterize 22 L. monocytogenes isolates of the WHOCCL collection with this PCR IVb variant 1 (IVb-v1) profile. The 22 isolates belong to the clinically predominant serovar 4b, exhibit 6 distinct pulsed-field gel electrophoresis ApaI/AscI combined profiles, and belong to 2 unrelated multilocus sequence types, indicating that the novel profile does not correspond to a recent clonal emergence. We have updated the WHOCCL serogroup-related PCR typing scheme to include this new profile.
世界卫生组织李斯特菌协作中心（WHOCCL）于 2004 年开发了一种多重 PCR 检测方法，可将 4 种主要的李斯特菌血清型（1/2a、1/2b、1/2c 和 4b）分为不同的 PCR 血清群。最近，已经确定了一种新的 PCR 图谱，由 prs、ORF2819、ORF2110 和 lmo0737 的扩增 DNA 片段组成。在此，我们用这种 PCR IVb 变体 1（IVb-v1）图谱对 WHOCCL 收集的 22 株李斯特菌进行了鉴定。这 22 株菌属于临床上主要的血清型 4b，表现出 6 种不同的脉冲场凝胶电泳 ApaI/AscI 组合图谱，并且属于 2 种不相关的多位点序列类型，表明该新图谱并不对应于近期的克隆出现。我们已经更新了 WHOCCL 与血清群相关的 PCR 分型方案，以包括这种新图谱。
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