Public Health Microbiology Laboratory, Queensland Health Forensic and Scientific Services, 39 Kessels Road, Coopers Plains, Queensland 4108, Australia.
J Clin Microbiol. 2011 Jan;49(1):426-9. doi: 10.1128/JCM.01207-10. Epub 2010 Nov 3.
Molecular serogroup-related PCR typing has made the determination of serotypes of Listeria monocytogenes isolates easy and rapid. Amplification of selected lineage- and serotype-related genes can produce serotype patterns reflecting the four major serotypes, 1/2a, 1/2b, 1/2c, and 4b. We found that four isolates in our routine testing had a pattern with the four bands lmo0737, ORF2110, ORF2819, and prs positive, a pattern which has not been previously reported in the literature. After testing with a lineage-specific PCR, hybridization, and conventional agglutination serotyping, the isolates with the new pattern were considered to be serotype 4b.
分子血清群相关 PCR 分型使得李斯特菌分离株血清型的确定变得简单而快速。选择谱系和血清型相关基因的扩增可以产生反映四个主要血清型 1/2a、1/2b、1/2c 和 4b 的血清型模式。我们发现，在我们的常规检测中，有四个分离株具有 lmo0737、ORF2110、ORF2819 和 prs 阳性的四个条带的模式，这一模式在文献中尚未报道过。在用谱系特异性 PCR、杂交和常规凝集血清分型进行测试后，具有新模式的分离株被认为是血清型 4b。
