鉴别腮腺炎病毒小疏水基因缺失对病毒毒力的影响与基因翻译对病毒毒力的影响。
Discrimination of mumps virus small hydrophobic gene deletion effects from gene translation effects on virus virulence.
机构信息
DVP/Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.
出版信息
J Virol. 2011 Jun;85(12):6082-5. doi: 10.1128/JVI.02686-10. Epub 2011 Apr 6.
Abstract
Deletion of the small hydrophobic (SH) protein of certain paramyxoviruses has been found to result in attenuation, suggesting that the SH protein is a virulence factor. To investigate the role of the mumps virus (MuV) SH protein in virulence, multiple stop codons were introduced into the open reading frame (ORF) of a MuV molecular clone (r88-1961(SHstop)), preserving genome structure but precluding production of the SH protein. No differences in neurovirulence were seen between the wild-type and the SH(stop) viruses. In contrast, upon deletion of the SH gene, significant neuroattenuation was observed. These data indicate that the MuV SH protein is not a neurovirulence factor and highlight the importance of distinguishing gene deletion effects from protein-specific effects.
摘要
某些副黏病毒的小疏水 (SH) 蛋白缺失已被发现会导致减毒，表明 SH 蛋白是一种毒力因子。为了研究腮腺炎病毒 (MuV) SH 蛋白在毒力中的作用，在 MuV 分子克隆 (r88-1961(SHstop)) 的开放阅读框 (ORF) 中引入了多个终止密码子，从而保留了基因组结构，但阻止了 SH 蛋白的产生。野生型和 SH(stop)病毒在神经毒力方面没有差异。相比之下，当 SH 基因缺失时，观察到明显的神经减毒作用。这些数据表明 MuV SH 蛋白不是神经毒力因子，并强调了区分基因缺失效应和蛋白特异性效应的重要性。
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