• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

胰岛细胞中环磷酸腺苷反应元件对大鼠胰高血糖素基因的转录激活作用。

Transcriptional activation of the rat glucagon gene by the cyclic AMP-responsive element in pancreatic islet cells.

作者信息

Knepel W, Chafitz J, Habener J F

机构信息

Laboratory of Molecular Endocrinology, Massachusetts General Hospital, Boston.

出版信息

Mol Cell Biol. 1990 Dec;10(12):6799-804. doi: 10.1128/mcb.10.12.6799-6804.1990.

DOI:10.1128/mcb.10.12.6799-6804.1990
PMID:2147227
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC362961/
Abstract

The 5'-flanking region of the rat glucagon gene contains, from nucleotides -291 to -298, a sequence (TGA CGTCA) which mediates cyclic AMP (cAMP) responsiveness in several genes (cAMP-responsive element [CRE]). However, because of nonpermissive bases surrounding the CRE octamer, the glucagon CRE does not confer cAMP responsiveness to an inert heterologous promoter in placental JEG cells that do not express the glucagon gene. This report describes transient transfection experiments with glucagon-reporter fusion genes that show that glucagon gene expression is activated by cAMP-dependent protein kinase A in a glucagon-expressing pancreatic islet cell line. This activation is mediated through the glucagon CRE.

摘要

大鼠胰高血糖素基因的5'侧翼区在核苷酸-291至-298处包含一个序列（TGA CGTCA），该序列在多个基因中介导环磷酸腺苷（cAMP）反应性（cAMP反应元件[CRE]）。然而，由于CRE八聚体周围存在非允许碱基，胰高血糖素CRE不能赋予不表达胰高血糖素基因的胎盘JEG细胞中惰性异源启动子cAMP反应性。本报告描述了使用胰高血糖素-报告基因融合基因进行的瞬时转染实验，结果表明在表达胰高血糖素的胰岛细胞系中，cAMP依赖性蛋白激酶A可激活胰高血糖素基因表达。这种激活是通过胰高血糖素CRE介导的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6db6/362961/f8aeea939674/molcellb00048-0715-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6db6/362961/96e69e6007a6/molcellb00048-0712-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6db6/362961/f8aeea939674/molcellb00048-0715-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6db6/362961/96e69e6007a6/molcellb00048-0712-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6db6/362961/f8aeea939674/molcellb00048-0715-a.jpg

相似文献

1
Transcriptional activation of the rat glucagon gene by the cyclic AMP-responsive element in pancreatic islet cells.胰岛细胞中环磷酸腺苷反应元件对大鼠胰高血糖素基因的转录激活作用。
Mol Cell Biol. 1990 Dec;10(12):6799-804. doi: 10.1128/mcb.10.12.6799-6804.1990.
2
Transcription of the rat glucagon gene by the cyclic AMP response element-binding protein CREB is modulated by adjacent CREB-associated proteins.环磷酸腺苷反应元件结合蛋白（CREB）对大鼠胰高血糖素基因的转录受到相邻CREB相关蛋白的调节。
Mol Cell Biol. 1993 Nov;13(11):7080-90. doi: 10.1128/mcb.13.11.7080-7090.1993.
3
Distinct properties of the cAMP-responsive element of the rat insulin I gene.大鼠胰岛素I基因cAMP反应元件的独特性质
J Biol Chem. 1994 Oct 28;269(43):27036-44.
4
Membrane depolarization and calcium influx induce glucagon gene transcription in pancreatic islet cells through the cyclic AMP-responsive element.膜去极化和钙内流通过环磷酸腺苷反应元件诱导胰岛细胞中胰高血糖素基因转录。
J Biol Chem. 1993 Mar 5;268(7):5168-77.
5
Somatostatin gene expression in pancreatic islet cells is directed by cell-specific DNA control elements and DNA-binding proteins.胰岛细胞中生长抑素基因的表达由细胞特异性DNA控制元件和DNA结合蛋白所调控。
J Biol Chem. 1989 Jun 15;264(17):10048-56.
6
The rat glucagon gene is regulated by a protein kinase A-dependent pathway in pancreatic islet cells.大鼠胰高血糖素基因在胰岛细胞中受蛋白激酶A依赖性途径调控。
Endocrinology. 1991 Jan;128(1):394-400. doi: 10.1210/endo-128-1-394.
7
Gene-specific transcriptional activity of the insulin cAMP-responsive element is conferred by NF-Y in combination with cAMP response element-binding protein.胰岛素cAMP反应元件的基因特异性转录活性由NF-Y与cAMP反应元件结合蛋白共同赋予。
J Biol Chem. 1998 Jul 17;273(29):18499-508. doi: 10.1074/jbc.273.29.18499.
8
Alpha-cell-specific expression of the glucagon gene is conferred to the glucagon promoter element by the interactions of DNA-binding proteins.通过DNA结合蛋白的相互作用，胰高血糖素基因的α细胞特异性表达被赋予到胰高血糖素启动子元件上。
Mol Cell Biol. 1988 Nov;8(11):4877-88. doi: 10.1128/mcb.8.11.4877-4888.1988.
9
Cyclic AMP-dependent protein kinase regulates transcription of the phosphoenolpyruvate carboxykinase gene but not binding of nuclear factors to the cyclic AMP regulatory element.环磷酸腺苷依赖性蛋白激酶调节磷酸烯醇式丙酮酸羧激酶基因的转录，但不调节核因子与环磷酸腺苷调节元件的结合。
Mol Cell Biol. 1990 Jul;10(7):3357-64. doi: 10.1128/mcb.10.7.3357-3364.1990.
10
Distinct adenosine 3',5'-monophosphate and phorbol ester-responsive signal transduction pathways converge at the level of transcriptional activation by the interactions of DNA-binding proteins.独特的3',5'-环磷酸腺苷和佛波酯反应信号转导途径通过DNA结合蛋白的相互作用在转录激活水平上汇聚。
Mol Endocrinol. 1989 May;3(5):868-80. doi: 10.1210/mend-3-5-868.

引用本文的文献

1
Intra-islet α-cell Gs signaling promotes glucagon release.胰岛内 α 细胞 Gs 信号转导促进胰高血糖素释放。
Nat Commun. 2024 Jun 15;15(1):5129. doi: 10.1038/s41467-024-49537-x.
2
Glucagon-like peptide 1 (GLP-1).胰高血糖素样肽 1（GLP-1）。
Mol Metab. 2019 Dec;30:72-130. doi: 10.1016/j.molmet.2019.09.010. Epub 2019 Sep 30.
3
Glucagon regulates its own synthesis by autocrine signaling.胰高血糖素通过自分泌信号调节自身的合成。

本文引用的文献

1
Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells.猿猴病毒40转化的仓鼠胰腺β细胞克隆细胞系中的胰岛素合成
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4339-43. doi: 10.1073/pnas.78.7.4339.
2
Adrenergic receptors and the secretion of glucagon and insulin from the isolated, perfused canine pancreas.肾上腺素能受体与离体灌注犬胰腺中胰高血糖素和胰岛素的分泌
J Clin Invest. 1973 Sep;52(9):2102-16. doi: 10.1172/JCI107395.
3
Firefly luciferase gene: structure and expression in mammalian cells.萤火虫荧光素酶基因：在哺乳动物细胞中的结构与表达
Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):20925-30. doi: 10.1073/pnas.1212870110. Epub 2012 Dec 3.
4
Direct regulation of the proglucagon gene by insulin, leptin, and cAMP in embryonic versus adult hypothalamic neurons.胰岛素、瘦素和环磷酸腺苷对胚胎期与成年期下丘脑神经元中胰高血糖素原基因的直接调控。
Mol Endocrinol. 2012 Aug;26(8):1339-55. doi: 10.1210/me.2012-1049. Epub 2012 Jun 5.
5
POU homeodomain protein Oct-1 functions as a sensor for cyclic AMP.POU 同源结构域蛋白 Oct-1 作为环磷酸腺苷（cAMP）的感受器发挥作用。
J Biol Chem. 2009 Sep 25;284(39):26456-65. doi: 10.1074/jbc.M109.030668. Epub 2009 Jul 18.
6
The role of incretins in glucose homeostasis and diabetes treatment.肠促胰岛素在葡萄糖稳态和糖尿病治疗中的作用。
Pharmacol Rev. 2008 Dec;60(4):470-512. doi: 10.1124/pr.108.000604. Epub 2008 Dec 12.
7
Characterization of a novel Foxa (hepatocyte nuclear factor-3) site in the glucagon promoter that is conserved between rodents and humans.胰高血糖素启动子中一个新型Foxa（肝细胞核因子-3）位点的特征分析，该位点在啮齿动物和人类之间保守。
Biochem J. 2005 Aug 1;389(Pt 3):831-41. doi: 10.1042/BJ20050334.
8
Protein hydrolysates stimulate proglucagon gene transcription in intestinal endocrine cells via two elements related to cyclic AMP response element.蛋白质水解产物通过与环磷酸腺苷反应元件相关的两个元件刺激肠道内分泌细胞中胰高血糖素原基因的转录。
Diabetologia. 2004 May;47(5):926-36. doi: 10.1007/s00125-004-1380-0. Epub 2004 Apr 14.
9
Overexpression of inducible cyclic AMP early repressor inhibits transactivation of genes and cell proliferation in pancreatic beta cells.诱导型环磷酸腺苷早期阻遏物的过表达抑制胰腺β细胞中基因的反式激活和细胞增殖。
Mol Cell Biol. 2004 Apr;24(7):2831-41. doi: 10.1128/MCB.24.7.2831-2841.2004.
10
Characterization of a novel protein kinase C response element in the glucagon gene.胰高血糖素基因中一种新型蛋白激酶C反应元件的特征分析
Mol Cell Biol. 1997 Apr;17(4):1805-16. doi: 10.1128/MCB.17.4.1805.
Mol Cell Biol. 1987 Feb;7(2):725-37. doi: 10.1128/mcb.7.2.725-737.1987.
4
Proglucagon gene expression and posttranslational processing in a hamster islet cell line.
Endocrinology. 1988 Oct;123(4):1861-7. doi: 10.1210/endo-123-4-1861.
5
Alpha-cell-specific expression of the glucagon gene is conferred to the glucagon promoter element by the interactions of DNA-binding proteins.通过DNA结合蛋白的相互作用，胰高血糖素基因的α细胞特异性表达被赋予到胰高血糖素启动子元件上。
Mol Cell Biol. 1988 Nov;8(11):4877-88. doi: 10.1128/mcb.8.11.4877-4888.1988.
6
Glucose regulates preproinsulin messenger RNA levels in a clonal cell line of simian virus 40-transformed B cells.葡萄糖调节猿猴病毒40转化的B细胞克隆细胞系中前胰岛素原信使核糖核酸水平。
FEBS Lett. 1987 Mar 9;213(1):149-54. doi: 10.1016/0014-5793(87)81481-3.
7
Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA.环磷酸腺苷反应性DNA结合蛋白：基于克隆的胎盘cDNA的结构
Science. 1988 Dec 9;242(4884):1430-3. doi: 10.1126/science.2974179.
8
Differentiation-induced gene expression in 3T3-L1 preadipocytes. Characterization of a differentially expressed gene encoding stearoyl-CoA desaturase.3T3-L1前脂肪细胞中分化诱导的基因表达。一种编码硬脂酰辅酶A去饱和酶的差异表达基因的特性。
J Biol Chem. 1988 Nov 25;263(33):17291-300.
9
Isolation of glucagon-secreting cell lines by cloning insulinoma cells.通过克隆胰岛素瘤细胞分离分泌胰高血糖素的细胞系。
In Vitro Cell Dev Biol. 1986 Mar;22(3 Pt 1):120-6. doi: 10.1007/BF02623498.
10
Interplay of nutrients and hormones in the regulation of glucagon release.
Endocrinology. 1985 Sep;117(3):817-23. doi: 10.1210/endo-117-3-817.