Faculdade de Medicina Veterinária-Univ Estadual Paulista-UNESP, Araçatuba, Brazil.
Parasitol Res. 2011 Sep;109(3):949-51. doi: 10.1007/s00436-011-2336-1. Epub 2011 Apr 7.
Cryptosporidiosis is a common protozoan disease observed in a wide range of vertebrate hosts, including ruminants. Cattle can be a potential reservoir of Cryptosporidium spp., leading to environmental contamination with oocysts of zoonotic species. The molecular characterization of Cryptosporidium spp. isolated from cattle from the state of São Paulo, Brazil, was accomplished using nested polymerase chain reaction for amplification of fragments of the 18S rRNA gene and the glycoprotein GP60 gene, following sequencing of amplified fragments. Positivity for Cryptosporidium was found in 10.7% (21/196) of the samples. Four species of Cryptosporidium were identified: C. andersoni, C. bovis, C. parvum subtype IIaA15G2R1, and C. ryanae. To the best of our knowledge, this is the first report of infection by C. ryanae and C. parvum IIaA15G2R1 in cattle from Brazil.
隐孢子虫病是一种常见的原生动物病，广泛存在于包括反刍动物在内的各种脊椎动物宿主中。牛可能是隐孢子虫属的潜在储主，导致其卵囊对人畜共患种造成环境污染。使用嵌套聚合酶链反应扩增 18S rRNA 基因和糖蛋白 GP60 基因的片段，并对扩增片段进行测序，对来自巴西圣保罗州的牛的隐孢子虫属进行了分子特征描述。在 196 个样本中，有 10.7%（21/196）的样本呈阳性。鉴定出了四种隐孢子虫：C. andersoni、C. bovis、C. parvum 亚型 IIaA15G2R1 和 C. ryanae。据我们所知，这是首次在巴西牛中发现感染 C. ryanae 和 C. parvum IIaA15G2R1 的报告。
