Fluorescence-enhanced potassium ions detection based on inherent quenching ability of deoxyguanosines and K(+)-induced conformational transition of G-rich ssDNA from duplex to G-quadruplex structures.

Herein, we develop a novel single fluorophore-labeled double-stranded oligonucleotide (OND) probe for rapid fluorescence-enhanced K(+) detection, based on an inherent quenching ability of guanine bases and G-rich OND conformation transition from duplex to G-quadruplex. This probe presents high sensitivity and good selectivity for the detection of K(+), and the assay process is simple and fast.