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一种用于柔性 FT-ICR 质谱成像的外部基质辅助激光解吸电离源，可对相邻样品进行内部校准。

An external matrix-assisted laser desorption ionization source for flexible FT-ICR Mass spectrometry imaging with internal calibration on adjacent samples.

机构信息

FOM Institute for Atomic and Molecular Physics, Science Park 104, 1098 XG Amsterdam, The Netherlands.

出版信息

J Am Soc Mass Spectrom. 2011 Jan;22(1):130-7. doi: 10.1007/s13361-010-0003-x. Epub 2011 Jan 20.

DOI:10.1007/s13361-010-0003-x
PMID:21472551
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3042104/
Abstract

We describe the construction and application of a new MALDI source for FT-ICR mass spectrometry imaging. The source includes a translational X-Y positioning stage with a 10×10 cm range of motion for analysis of large sample areas, a quadrupole for mass selection, and an external octopole ion trap with electrodes for the application of an axial potential gradient for controlled ion ejection. An off-line LC MALDI MS/MS run demonstrates the utility of the new source for data- and position-dependent experiments. A FT-ICR MS imaging experiment of a coronal rat brain section yields ∼200 unique peaks from m/z 400-1100 with corresponding mass-selected images. Mass spectra from every pixel are internally calibrated with respect to polymer calibrants collected from an adjacent slide.

摘要

我们描述了一种新的 MALDI 源的构建和应用，用于 FT-ICR 质谱成像。该源包括一个平移 X-Y 定位台，运动范围为 10×10cm，用于分析大的样品区域，一个四极杆用于质量选择，以及一个外部八极离子阱，带有用于施加轴向电势梯度以控制离子引出的电极。离线 LC MALDI MS/MS 运行证明了新源在数据和位置相关实验中的实用性。对冠状大鼠脑切片的 FT-ICR MS 成像实验产生了约 200 个独特的峰，质荷比范围为 400-1100，具有相应的质量选择图像。每个像素的质谱都与从相邻载玻片上收集的聚合物校准剂进行内部校准。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b8/3042104/896d39908406/13361_2010_3_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b8/3042104/6288a89e95f9/13361_2010_3_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b8/3042104/1a59bea6c723/13361_2010_3_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b8/3042104/c88f73c03824/13361_2010_3_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b8/3042104/b26cfbde3314/13361_2010_3_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b8/3042104/896d39908406/13361_2010_3_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b8/3042104/6288a89e95f9/13361_2010_3_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b8/3042104/1a59bea6c723/13361_2010_3_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b8/3042104/c88f73c03824/13361_2010_3_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b8/3042104/b26cfbde3314/13361_2010_3_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8b8/3042104/896d39908406/13361_2010_3_Fig5_HTML.jpg

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