Department of Chemistry, Purdue University, Brown Building, 560 Oval Drive, West Lafayette, IN 47906, USA.
J Am Soc Mass Spectrom. 2011 Mar;22(3):520-30. doi: 10.1007/s13361-010-0050-3. Epub 2011 Feb 1.
We report here a comparison of the use of diagnostic ion-molecule reactions for the identification of oxygen-containing functional groups in Fourier-transform ion cyclotron resonance (FTICR) and linear quadrupole ion trap (LQIT) mass spectrometers. The ultimate goal of this research is to be able to identify functionalities in previously unknown analytes by using many different types of mass spectrometers. Previous work has focused on the reactions of various boron reagents with protonated oxygen-containing analytes in FTICR mass spectrometers. By using a LQIT modified to allow the introduction of neutral reagents into the helium buffer gas, this methodology has been successfully implemented to this type of an ion trap instrument. The products obtained from the reactions of trimethyl borate (TMB) with various protonated analytes are compared for the two instruments. Finally, the ability to integrate these reactions into LC-MS experiments on the LQIT is demonstrated.
我们在此报告了在傅里叶变换离子回旋共振（FTICR）和线性四极离子阱（LQIT）质谱仪中，使用诊断离子-分子反应来鉴定含氧官能团的比较。这项研究的最终目标是能够通过使用许多不同类型的质谱仪来鉴定以前未知的分析物的功能。以前的工作集中在各种硼试剂与 FTICR 质谱仪中质子化含氧分析物的反应上。通过使用一种经过修改的 LQIT，可以将中性试剂引入氦缓冲气体中，这种方法已成功应用于这种类型的离子阱仪器。比较了两种仪器中三甲基硼酸（TMB）与各种质子化分析物反应得到的产物。最后，证明了能够将这些反应集成到 LQIT 上的 LC-MS 实验中。
