Department of Chemistry and Biochemistry, University of Texas, Austin, TX 78712, USA.
J Am Soc Mass Spectrom. 2011 Feb;22(2):197-206. doi: 10.1007/s13361-010-0023-6. Epub 2011 Jan 28.
The development of new ion activation/dissociation methods is motivated by the need for more versatile ways to characterize structures of ions, especially in the growing arena of biological mass spectrometry in which better tools for determining sequences, modifications, interactions, and conformations of biopolymers are essential. Although most agree that collision-induced dissociation (CID) remains the gold standard for ion activation/dissociation, recent inroads in electron- and photon-based activation methods have cemented their role as outstanding alternatives. This article will focus on the impact of photodissociation, including its strengths and drawbacks as an analytical tool, and its potential for further development in the next decade. Moreover, the discussion will emphasize photodissociation in quadrupole ion traps, because that platform has been used for one of the greatest arrays of new applications over the past decade.
新的离子活化/解离方法的发展是出于需要更通用的方法来描述离子的结构，特别是在生物质谱学这个不断发展的领域，其中更好的工具对于确定生物聚合物的序列、修饰、相互作用和构象是至关重要的。尽管大多数人都认为碰撞诱导解离（CID）仍然是离子活化/解离的金标准，但近年来电子和光子基活化方法的进展已经巩固了它们作为出色替代品的地位。本文将重点讨论光解离的影响，包括它作为分析工具的优缺点，以及它在未来十年进一步发展的潜力。此外，讨论将强调在四极离子阱中的光解离，因为在过去十年中，该平台已经被用于许多新的应用。
