Kao M C, French B A, Chang S H, Ho C F
Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan, ROC.
Proc Natl Sci Counc Repub China B. 1990 Apr;14(2):69-74.
A partial cDNA for rabbit muscle phosphofructokinase (Rm-PFK) has been cloned and sequenced. The nucleotide sequence of the cDNA agrees with the previously determined Rm-PFK genomic sequence. In addition, the amino acid sequence deduced from the cDNA is nearly identical to the Rm-PFK sequence previously determined by peptide analysis. A significant degree of homology exists when the amino acid sequence of Rm-PFK is compared with the sequences of Bacillus stearothermophilus PFK or Escherichia coli PFK-1. The cloning and sequencing of the PFK cDNA fragment represents significant progress toward the long term goal of using site-specific mutagenesis to investigate the structure-function relationships in this allosteric enzyme.
兔肌肉磷酸果糖激酶（Rm-PFK）的部分互补DNA（cDNA）已被克隆并测序。该cDNA的核苷酸序列与先前确定的Rm-PFK基因组序列一致。此外，从cDNA推导的氨基酸序列与先前通过肽分析确定的Rm-PFK序列几乎相同。当将Rm-PFK的氨基酸序列与嗜热脂肪芽孢杆菌PFK或大肠杆菌PFK-1的序列进行比较时，存在显著程度的同源性。PFK cDNA片段的克隆和测序代表了朝着利用位点特异性诱变研究这种别构酶的结构-功能关系这一长期目标迈出的重要进展。
