Daldal F
Gene. 1984 Jun;28(3):337-42. doi: 10.1016/0378-1119(84)90151-3.
The nucleotide sequence of a 1.3-kb DNA fragment containing the entire pfkB gene which codes for Pfk-2 of Escherichia coli, a minor phosphofructokinase (Pfk) enzyme, is reported. The Pfk-2 protein subunit is encoded by 924 bp, has 308 amino acids and an Mr of 33 000. Like other weakly expressed E. coli genes the codon usage in the pfkB gene is random; there is no strong bias for the usage of major tRNA isoaccepting species, and the codon preference rules of Grosjean and Fiers [Gene, 18 (1982) 199-209] are followed. This is the first report of the complete gene sequence of a phosphofructokinase.
报道了一个1.3kb DNA片段的核苷酸序列，该片段包含编码大肠杆菌次要磷酸果糖激酶（Pfk）即Pfk-2的完整pfkB基因。Pfk-2蛋白亚基由924bp编码，有308个氨基酸，分子量为33000。与其他低表达的大肠杆菌基因一样，pfkB基因中的密码子使用是随机的；对主要同功tRNA种类的使用没有强烈偏好，并且遵循Grosjean和Fiers [《基因》，18 (1982) 199 - 209]的密码子偏好规则。这是磷酸果糖激酶完整基因序列的首次报道。
