单克隆抗体A7-新制癌菌素缀合物（A7-NCS）在靶细胞中的结合、内化及细胞毒性
Binding, internalization and the cytotoxicity of monoclonal antibody A7-neocarzinostatin conjugates (A7-NCS) in target cells.
作者信息
Kitamura K, Takahashi T, Noguchi A, Takashina K, Tsurumi H, Yamaguchi T
机构信息
First Department of Surgery, Kyoto Prefectural University of Medicine.
出版信息
Tohoku J Exp Med. 1990 Jul;161(3):199-207. doi: 10.1620/tjem.161.199.
To study the mechanism of action of the monoclonal antibody A7-neocarzinostatin conjugates (A7-NCS), the internalization of the antibody and its conjugate into target cells was examined. The incubation of radiolabeled A7 and A7-NCS with target cells revealed that both were taken up by target cells in a time dependent fashion. The immunocytochemical study using anti-NCS also revealed the intracellular localization of the conjugates. The cytotoxicity of the conjugate was markedly reduced when the binding sites were occupied by an excess of antibody on the cell surface. These results showed that A7-NCS was internalized into target cells and that its cytotoxicity was mediated through specific binding and internalization.
为研究单克隆抗体A7-新制癌菌素缀合物（A7-NCS）的作用机制，对抗体及其缀合物在靶细胞中的内化进行了检测。用放射性标记的A7和A7-NCS与靶细胞孵育，结果显示二者均以时间依赖性方式被靶细胞摄取。使用抗NCS的免疫细胞化学研究也揭示了缀合物在细胞内的定位。当细胞表面的结合位点被过量抗体占据时，缀合物的细胞毒性显著降低。这些结果表明，A7-NCS被内化到靶细胞中，其细胞毒性是通过特异性结合和内化介导的。
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