Zacharczuk Katarzyna, Gierczyński Rafał
Zakład Bakteriologii Narodowego Instytutu Zdrowia Publicznego-Państwowego Zakładu Higieny w Warszawie.
Med Dosw Mikrobiol. 2010;62(4):331-6.
In our study we analyzed the nucleotide sequence of the C- terminal 256 bp fragment of the myfA gene encoding MyfA protein, the major subunit of Yersinia enterocolitica Myf fimbriae. We examined ten representative strains of major Y. enterocolitica pathogenic bioserotypes belonging to European (4/O3; 2/O9; 3/O5,27) and American (1B/O8) phylogenetic lineages. DNA sequencing revealed that consensus nucleotide sequences of the tested myfA fragment were indistinguishable in all the tested strains. The resulting common consensus sequence found in our study was identical to the corresponding fragment of reference sequences Z21953 and NC008800 deposited in GenBank database for pathogenic Y. enterocolitica strains. In contrast, 18 point mutations leading to 13 amino acid substitutions were found when the common consensus sequence was aligned to sequence AY966879 determined for the myfA homologue detected by PCR in Y. enterocolitica 1A strain. The strong conservation of the nucleotide and amino acid sequence of myfA gene among virulent bioserotypes of Y. enterocolitica indicate that fimbriae MyF could play important role in pathogenesis, even before the divergence of European and American lineages.
在我们的研究中，我们分析了编码耶尔森氏菌小肠结肠炎耶尔森氏菌Myf菌毛主要亚基MyfA蛋白的myfA基因C端256 bp片段的核苷酸序列。我们检测了属于欧洲（4/O3；2/O9；3/O5,27）和美国（1B/O8）系统发育谱系的主要小肠结肠炎耶尔森氏菌致病生物血清型的十个代表性菌株。DNA测序显示，在所有测试菌株中，测试的myfA片段的共有核苷酸序列没有差异。我们研究中得到的共同共有序列与GenBank数据库中为致病性小肠结肠炎耶尔森氏菌菌株保存的参考序列Z21953和NC008800的相应片段相同。相比之下，当将共同共有序列与通过PCR在小肠结肠炎耶尔森氏菌1A菌株中检测到的myfA同源物确定的序列AY966879进行比对时，发现了18个导致13个氨基酸替换的点突变。小肠结肠炎耶尔森氏菌强毒株生物血清型中myfA基因核苷酸和氨基酸序列的高度保守表明，MyF菌毛甚至在欧美谱系分化之前就可能在发病机制中发挥重要作用。
