Zacharczuk Katarzyna
Med Dosw Mikrobiol. 2013;65(4):233-43.
The high-pathogenicity Y. enterocolitica bioserotype 1B/O8 has been isolated from human clinical samples since 2004 year in Poland. The group of "American" strains of Y. enterocolitica is considered to be the second, major causative agent ofyersiniosis in Poland, after the predominant bioserotype 4/O3. The high-pathogenicity of Y. enterocolitica 1B/O8 is likely attributed to the presence of a couple of chromosomally encoded virulence factors including yersiniabactin (Ybt). The aim of the present study was to examine the occurrence of known virulence factors in human clinical isolates of Yersinia enterocolitica bioserotype 18/08, isolated in Poland.
The group of 64 isolates of Y. enterocolitica bioserotype 1B/O8, isolated from clinical specimens in Poland in 2009 year was examined for the presence of the selected virulence determinants by multiplex-PCR.
All of the tested Y. enterocolitica 1B/O8 isolates have the same virulotype (ail+, ystA+, myfA+, myfB+, myfC+ irp1+, irp2+, fuyA+, yst1+ chiY+, ysrS+), specific for the high--pathogenicity American strains of Y. enterocolitica.
Presence of the genes encoding yersiniabactin (irp1+, irp2+, fuyA+), chromosomal secretion system Ysa (chiY+, ysrS+) and Yst1 (yst1+) may argue for the extended pathogenic potential of the Y. enterocolitica bioserotype 1B/O8 in Poland.
自2004年以来，高致病性小肠结肠炎耶尔森菌生物血清型1B/O8已从波兰的人类临床样本中分离出来。小肠结肠炎耶尔森菌“美国”菌株组被认为是波兰耶尔森菌病的第二大主要病原体，仅次于占主导地位的生物血清型4/O3。小肠结肠炎耶尔森菌1B/O8的高致病性可能归因于几个染色体编码的毒力因子的存在，包括耶尔森菌素（Ybt）。本研究的目的是检测在波兰分离的小肠结肠炎耶尔森菌生物血清型18/08的人类临床分离株中已知毒力因子的存在情况。
对2009年从波兰临床标本中分离出的64株小肠结肠炎耶尔森菌生物血清型1B/O8菌株进行多重聚合酶链反应，检测所选毒力决定因素的存在情况。
所有测试的小肠结肠炎耶尔森菌1B/O8分离株具有相同的毒力型（ail +，ystA +，myfA +，myfB +，myfC +，irp1 +，irp2 +，fuyA +，yst1 +，chiY +，ysrS +），这是高致病性小肠结肠炎耶尔森菌美国菌株所特有的。
编码耶尔森菌素的基因（irp1 +，irp2 +，fuyA +）、染色体分泌系统Ysa（chiY +，ysrS +）和Yst1（yst1 +）的存在可能表明小肠结肠炎耶尔森菌生物血清型1B/O8在波兰具有更大的致病潜力。
