Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Tokyo, 113-0033, Japan.
J Am Chem Soc. 2011 May 4;133(17):6745-51. doi: 10.1021/ja200225m. Epub 2011 Apr 7.
We present a fluorescence activation-coupled protein labeling (FAPL) method, which employs small-molecular probes that exhibit almost no basal fluorescence but acquire strong fluorescence upon covalent binding to tag-proteins. This method enables real-time imaging of protein labeling without any washout process and is uniquely suitable for real-time imaging of protein dynamics on the cell surface. We applied this method to address the spatiotemporal dynamics of the EGF receptor during cell migration.
我们提出了一种荧光激活偶联蛋白标记（FAPL）方法，该方法使用小分子探针，这些探针在与标记蛋白共价结合之前几乎没有本底荧光，但结合后会获得强荧光。这种方法可以在无需任何洗脱过程的情况下实现蛋白质标记的实时成像，特别适合用于实时成像细胞表面上蛋白质的动力学。我们应用该方法来研究细胞迁移过程中表皮生长因子受体的时空动力学。
