O'Hare Helen M, Johnsson Kai, Gautier Arnaud
Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.
Curr Opin Struct Biol. 2007 Aug;17(4):488-94. doi: 10.1016/j.sbi.2007.07.005. Epub 2007 Sep 11.
Site-specific protein labeling with synthetic dyes is an emerging technique for live cell imaging. A protein or peptide tag fused to the protein of interest provides the means for attachment of a fluorophore or other small molecule probe, to allow non-invasive imaging of the dynamics of protein localization. The past two years have seen significant advances in such methods, the publication of a number of new tags for labeling, and the imaginative application of established techniques to tackle previously intractable biological questions.
用合成染料进行位点特异性蛋白质标记是一种新兴的活细胞成像技术。与目标蛋白融合的蛋白质或肽标签为荧光团或其他小分子探针的附着提供了途径，从而能够对蛋白质定位动态进行非侵入性成像。在过去两年中，此类方法取得了重大进展，出现了许多用于标记的新标签，并将成熟技术进行了创新性应用以解决以前难以处理的生物学问题。
