State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou, Guangdong, China.
Gen Comp Endocrinol. 2011 Jul 1;172(3):371-81. doi: 10.1016/j.ygcen.2011.03.025. Epub 2011 Apr 5.
Estrogen plays key roles in vertebrate reproductive system via estrogen receptors (ERs) as mediating pathways. In the present study, three full-length ERs cDNA sequences were isolated from a protogynous teleost, the orange-spotted grouper (Epinephelus coioides), and were 2235bp for gERα, 1967bp for gERβ1 and 2158bp for gERβ2, respectively. Phylogenetic and amino acid alignment analyses showed that each gER was clustered in the corresponding taxonomic groups of the perciformes and exhibited high evolutional conservation in functional domains. RT-PCR revealed that gERs expressed at different levels in all the obtained tissues. gERα highly expressed in mature ovaries, gERβ1 mainly expressed in immature ovaries and gERβ2 varied greatly during ovarian development. During female to male sex reversal induced by 17α-methyltestosterone (MT) implantation, gERα decreased gradually, gERβ1 increased gradually, and gERβ2 decreased firstly and recovered subsequently in male stage. The present study speculated the potential roles of gERs during female maturation and female to male sex reversal induced by MT in the protogynous grouper E. coioides.
雌激素通过雌激素受体（ERs）作为介导途径在脊椎动物生殖系统中发挥关键作用。本研究从雌雄同体的石斑鱼（Epinephelus coioides）中分离出三个全长 ERs cDNA 序列，它们分别为 gERα（2235bp）、gERβ1（1967bp）和 gERβ2（2158bp）。系统发育和氨基酸比对分析表明，每个 gER 都聚类在鲈形目相应的分类群中，并在功能域中表现出高度的进化保守性。RT-PCR 显示 gERs 在所有获得的组织中表达水平不同。gERα 在成熟卵巢中高度表达，gERβ1 主要在未成熟卵巢中表达，gERβ2 在卵巢发育过程中变化很大。在 17α-甲基睾丸酮（MT）植入诱导的雌性到雄性性别逆转期间，gERα 逐渐减少，gERβ1 逐渐增加，gERβ2 先减少后在雄性阶段恢复。本研究推测 gERs 在石斑鱼 E. coioides 的雌性成熟和 MT 诱导的雌性到雄性性别逆转过程中的潜在作用。
