Joint Diabetes, Endocrinology & Metabolism Program, Louisiana State University System, Louisiana State University Health Science Center-New Orleans, New Orleans, LA 70112, USA.
Peptides. 2011 Jun;32(6):1270-5. doi: 10.1016/j.peptides.2011.03.022. Epub 2011 Apr 5.
Pyroglutamylated arginine-phenylalanineamide peptide (QRFP) is a neuropeptide involved in feeding behavior. Central administration of QRFP selectively increases the intake of a high fat diet in male rats. QRFP administration also stimulates the hypothalamic-pituitary-gonadal axis via gonadotrophin-releasing hormone in male and female rats. Prepro-QRFP mRNA is expressed in localized regions of the mediobasal hypothalamus which are abundant in neurotransmitters, neuropeptides and receptor systems important for food intake regulation and reproductive behaviors. The current experiments were conducted to investigate the effects of centrally administered QRFP-26 on the intake of a high fat diet (HFD, 60%kcal from fat) in female rats and to investigate alterations in hypothalamic prepro-QRFP and its receptors, GPR130a and GPR103b, mRNA levels over the estrous cycle. In Experiment 1, female rats were administered QRFP-26 (intracerebroventricular; 0.3nmol, 0.5nmol, 1.0nmol) in rats consuming either a HFD or a low fat diet. All doses of QRFP-26 selectively increased the intake of the HFD in female rats. These data suggest that QRFP-26 regulates the intake of energy dense foods in female rats, which is similar to previous findings in male rats. In Experiment 2, hypothalamic levels of prepro-QRFP mRNA and its receptors were assessed during diestrus, proestrus, or estrus. The level of prepro-QRFP mRNA in the ventromedial/arcuate nucleus (VMH/ARC) of the hypothalamus was increased during proestrus, which suggests that endogenous estrogen levels regulate QRFP expression in the VMH/ARC. These data suggest that QRFP may play a role in coordinating feeding behaviors with reproductive function when energy demand is increased.
焦谷氨酸-精氨酸-苯丙酰胺肽（QRFP）是一种参与摄食行为的神经肽。中央给予 QRFP 选择性地增加雄性大鼠高脂肪饮食的摄入量。QRFP 给药还通过雄性和雌性大鼠中的促性腺激素释放激素刺激下丘脑-垂体-性腺轴。前 QRFP mRNA 在中脑基底部下丘脑的局部区域表达，这些区域富含神经递质、神经肽和受体系统，对于调节摄食和生殖行为非常重要。目前的实验旨在研究中枢给予 QRFP-26 对雌性大鼠高脂肪饮食（HFD，60%卡路里来自脂肪）摄入量的影响，并研究下丘脑前 QRFP 及其受体 GPR130a 和 GPR103b 在发情周期中的 mRNA 水平的变化。在实验 1 中，雌性大鼠给予 QRFP-26（侧脑室；0.3nmol、0.5nmol、1.0nmol），同时大鼠摄入 HFD 或低脂饮食。QRFP-26 的所有剂量都选择性地增加了雌性大鼠对 HFD 的摄入量。这些数据表明，QRFP-26 调节雌性大鼠对能量密集型食物的摄入量，这与以前在雄性大鼠中的发现相似。在实验 2 中，评估了发情周期中下丘脑前 QRFP mRNA 及其受体的水平。下丘脑腹内侧/弓状核（VMH/ARC）中的前 QRFP mRNA 水平在发情前期增加，这表明内源性雌激素水平调节 VMH/ARC 中的 QRFP 表达。这些数据表明，当能量需求增加时，QRFP 可能在协调摄食行为与生殖功能方面发挥作用。
