单细胞抗体技术。
Single B cell antibody technologies.
机构信息
Max Planck Institute for Infection Biology, D-10117 Berlin, Germany.
出版信息
N Biotechnol. 2011 Sep;28(5):453-7. doi: 10.1016/j.nbt.2011.03.014. Epub 2011 Apr 5.
Abstract
Monoclonal antibodies (mAbs) are arguably the most significant class of biologics for use as pharmaceuticals and diagnostics. Many technological concepts exist for the generation and identification of therapeutically relevant mAbs, including the isolation and cloning of immunoglobulin (Ig) encoding genes from single B-lineage cells. This review summarizes various single B cell approaches and describes their use for the discovery of mAbs with potential therapeutic values or in basic research.
摘要
单克隆抗体 (mAbs) 可以说是作为药物和诊断试剂使用的最重要的一类生物制剂。存在许多用于产生和鉴定治疗相关 mAbs 的技术概念，包括从单个 B 细胞谱系细胞中分离和克隆免疫球蛋白 (Ig) 编码基因。本综述总结了各种单 B 细胞方法，并描述了它们在发现具有潜在治疗价值的 mAbs 或在基础研究中的应用。
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