Krüger D, Koch J, Barthelmess I B
Institut für Angewandte Genetik, Universität Hannover, Federal Republic of Germany.
Curr Genet. 1990 Oct;18(3):211-5. doi: 10.1007/BF00318383.
In Neurospora crassa starvation for single amino acids leads to derepression of enzymes in many amino acid synthetic pathways. Regulation occurs at the level of transcription via "general amino acid (or cross-pathway) control". In this paper a new regulatory gene, cpc-2, is described that specifies a positive, trans-acting effector involved in this control. This gene, located on linkage group VII, was identified by a recessive mutation, U142, which results in sensitivity for two amino acid analogues and a lack of enzyme derepression in response to amino acid limitation. It was shown that cpc-2 (U142) impairs the activation of transcription of amino acid structural genes in several biosyntheses. The only other known regulatory gene involved in general amino acid control of Neurospora is cpc-1. Transcription of the cpc-1 gene, however, is increased in response to amino acid starvation irrespective of the presence of the mutation U142.
在粗糙脉孢菌中，单一氨基酸饥饿会导致许多氨基酸合成途径中的酶去阻遏。调节通过“一般氨基酸（或交叉途径）控制”在转录水平上发生。本文描述了一个新的调节基因cpc - 2，它指定了一种参与这种控制的正向反式作用效应物。这个位于连锁群VII上的基因是通过隐性突变U142鉴定出来的，该突变导致对两种氨基酸类似物敏感，并且在氨基酸限制时缺乏酶去阻遏。结果表明，cpc - 2（U142）损害了几种生物合成中氨基酸结构基因转录的激活。粗糙脉孢菌中参与一般氨基酸控制的唯一其他已知调节基因是cpc - 1。然而，无论突变U142是否存在，cpc - 1基因的转录在氨基酸饥饿时都会增加。
