蛋白质周转的动态状态：是时候了。

The dynamic state of protein turnover: It's about time.

机构信息

Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA.

出版信息

Trends Cell Biol. 2011 May;21(5):293-303. doi: 10.1016/j.tcb.2011.02.002. Epub 2011 Apr 7.

DOI:10.1016/j.tcb.2011.02.002

Abstract

The continual destruction and renewal of proteins that maintain cellular homeostasis has been rigorously studied since the late 1930s. Experimental techniques for measuring protein turnover have evolved to measure the dynamic regulation of key proteins and now, entire proteomes. In the past decade, the proteomics field has aimed to discover how cells adjust their proteomes to execute numerous regulatory programs in response to specific cellular and environmental cues. By combining classical biochemical techniques with modern, high-throughput technologies, researchers have begun to reveal the synthesis and degradation mechanisms that shape protein turnover on a global scale. This review examines several recent developments in protein turnover research, emphasizing the combination of metabolic labeling and mass spectrometry.

摘要

自 20 世纪 30 年代末以来，人们一直在深入研究蛋白质的持续破坏和更新，以维持细胞内环境稳定。用于测量蛋白质周转率的实验技术已经发展到可以测量关键蛋白质甚至整个蛋白质组的动态调节。在过去的十年中，蛋白质组学领域的目标是发现细胞如何调整其蛋白质组，以响应特定的细胞和环境线索执行众多调节程序。通过将经典生化技术与现代高通量技术相结合，研究人员已经开始揭示在全局范围内影响蛋白质周转率的合成和降解机制。本文综述了蛋白质周转率研究的几个最新进展，重点介绍了代谢标记和质谱联用的方法。