Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan.
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.
Int J Mol Sci. 2024 May 28;25(11):5882. doi: 10.3390/ijms25115882.
Global warming poses a threat to plant survival, impacting growth and agricultural yield. Protein turnover, a critical regulatory mechanism balancing protein synthesis and degradation, is crucial for the cellular response to environmental changes. We investigated the effects of elevated temperature on proteome dynamics in seedlings using N-stable isotope labeling and ultra-performance liquid chromatography-high resolution mass spectrometry, coupled with the algorithm. Analyzing different cellular fractions from plants grown under 22 °C and 30 °C growth conditions, we found significant changes in the turnover rates of 571 proteins, with a median 1.4-fold increase, indicating accelerated protein dynamics under thermal stress. Notably, soluble root fraction proteins exhibited smaller turnover changes, suggesting tissue-specific adaptations. Significant turnover alterations occurred with redox signaling, stress response, protein folding, secondary metabolism, and photorespiration, indicating complex responses enhancing plant thermal resilience. Conversely, proteins involved in carbohydrate metabolism and mitochondrial ATP synthesis showed minimal changes, highlighting their stability. This analysis highlights the intricate balance between proteome stability and adaptability, advancing our understanding of plant responses to heat stress and supporting the development of improved thermotolerant crops.
全球变暖对植物生存构成威胁，影响其生长和农业产量。蛋白质周转是一种平衡蛋白质合成和降解的关键调控机制，对于细胞对环境变化的响应至关重要。我们使用 N-稳定同位素标记和超高效液相色谱-高分辨率质谱联用技术以及 算法，研究了高温对 幼苗蛋白质组动态的影响。分析在 22°C 和 30°C 生长条件下生长的植物的不同细胞分数，我们发现 571 种蛋白质的周转率有显著变化，中位数增加了 1.4 倍，表明在热应激下蛋白质动力学加速。值得注意的是，可溶性根部分蛋白质的周转率变化较小，表明存在组织特异性适应。氧化还原信号、应激反应、蛋白质折叠、次生代谢和光呼吸等方面的显著周转率变化表明，复杂的响应增强了植物的耐热性。相反，参与碳水化合物代谢和线粒体 ATP 合成的蛋白质变化最小，突出了它们的稳定性。这项分析强调了蛋白质组稳定性和适应性之间的复杂平衡，增进了我们对植物对热应激响应的理解，并支持开发改良的耐热作物。
