Al-Ghasham Abdalla, Ata Hesham Saad, El-Deep Said, Meki Abdel-Raheim, Shehada Salah
Department of Pharmacology, College of Medicine, Qassim University, Saudi Arabia.
Int J Health Sci (Qassim). 2008 Jul;2(2):26-44.
Many medicinal plants and their purified constituents have been shown beneficial therapeutic potentials. Seeds of Nigella sativa, a dicotyledon of the Ranunculaceae family, have been utilized for thousands of years as a spice and food preservative.
the toxic effect of aflatoxin-B(1) (AFB(1)) and the possible cytoprotective effect of Nigella sativa (NS) oil and aqueous extract of date were studied on 40 male rats. The animals were divided into 4 groups (10 rats each) and treated daily for two weeks. Group 1 received normal saline as controls. Group 2 treated via intraperitoneal (IP) route with AFB(1) (50μg/kg BW). Group 3 treated with AFB(1) and NS oil via IP. Group 4 treated with AFB(1) and received orally aqueous extract of date (15mg/15ml). The liver and kidneys of each animal were histological examined and biochemical evaluation of the liver and kidney functions was performed.
Group 2 showed severe degenerative and necrotic changes in the liver and kidney. The plasma levels of alanine transaminase (ALT), aspartate transaminase (AST), creatinine and urea in AFB(1) group were significantly higher than the control group. Livers and kidneys of rats, treated with AFB(1) and NS showed less histopathological changes in comparison with the AFB(1) treated group. Livers and kidneys of rats treated with AFB1 and date group showed only mild histopathological changes in comparison with AFB(1) treated group. These histopathological changes seen in animals treated with AFB1 and dates were associated with a significant reduction in levels of ALT, AST, creatinine and urea. Likewise, histopathological changes in the AFB1 and NS group were associated with significant reduction in the levels of beforementioned indices. Moreover, AFB1 and date group showed significant improvement in liver function comparing with AFB(1) and NS group.
our study revealed that treatment with AFB(1) induced histopathological changes in the tissues of liver and kidney associated with dysfunction of these organs. Both NS and date reduce the toxic effects of AFB(1) in liver and kidney. But date treatment was more cytoprotective for liver than NS treatment against aflatoxicosis in rats.
许多药用植物及其纯化成分已显示出有益的治疗潜力。黑种草是毛茛科的双子叶植物，其种子作为香料和食品防腐剂已被使用了数千年。
研究了黄曲霉毒素B1（AFB1）的毒性作用以及黑种草（NS）油和枣水提取物对40只雄性大鼠的可能细胞保护作用。将动物分为4组（每组10只大鼠），并每天治疗两周。第1组接受生理盐水作为对照。第2组通过腹腔注射（IP）途径用AFB1（50μg/kg体重）治疗。第3组通过IP用AFB1和NS油治疗。第4组用AFB1治疗并口服枣水提取物（15mg/15ml）。对每只动物的肝脏和肾脏进行组织学检查，并对肝功能和肾功能进行生化评估。
第2组在肝脏和肾脏中显示出严重的退行性和坏死性变化。AFB1组中的丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、肌酐和尿素的血浆水平显著高于对照组。与AFB1治疗组相比，用AFB1和NS治疗的大鼠的肝脏和肾脏显示出较少的组织病理学变化。与AFB1治疗组相比，用AFB1和枣治疗的大鼠的肝脏和肾脏仅显示出轻度的组织病理学变化。在用AFB1和枣治疗的动物中看到的这些组织病理学变化与ALT、AST、肌酐和尿素水平的显著降低有关。同样，AFB1和NS组中的组织病理学变化与上述指标水平的显著降低有关。此外，与AFB1和NS组相比，AFB1和枣组在肝功能方面显示出显著改善。
我们的研究表明，用AFB1治疗会诱导肝脏和肾脏组织中的组织病理学变化，并伴有这些器官的功能障碍。NS和枣均可降低AFB1对肝脏和肾脏的毒性作用。但在大鼠抗黄曲霉毒素中毒方面，枣治疗对肝脏的细胞保护作用比NS治疗更强。
