Hansen Torben F, Garm Spindler Karen-Lise, Lorentzen Karen A, Olsen Dorte A, Andersen Rikke F, Lindebjerg Jan, Brandslund Ivan, Jakobsen Anders
Department of Oncology, Vejle Hospital, 7100 Vejle, Denmark.
Mol Med Rep. 2009 Sep-Oct;2(5):787-92. doi: 10.3892/mmr_00000173.
Vascular endothelial growth factor A (VEGF-A) is a key regulator of angiogenesis that binds to the receptors VEGFR-1 and VEGFR-2. It is well known that VEGF-A levels are increased in colorectal cancer (CRC) tissue compared to normal colorectal tissue, but little is known regarding the expression of the VEGFR-1 and -2 receptors. The aim of the present study was to perform a quantitative analysis of VEGFR-1 and -2 at the mRNA and protein level in tumour and normal colorectal tissue from CRC patients. Tissues were collected from 110 patients who underwent surgical resection for CRC. Receptor status was analysed at the gene expression level by real time RT-PCR using β-2-microglobulin and β-actin as reference genes. Protein analysis was performed using the ELISA technique. Gene expression and protein concentrations of VEGFR-1 and -2 were significantly increased in CRC tissue compared to normal colorectal tissue (P<10-6 for both receptors). An association between the gene expression of VEGFR-2 in CRC tissue and disease stage was detected (P=0.055). Significant correlations were also found between the gene expression of VEGFR-1 and -2 in CRC tissue and normal colorectal tissue (P<10-4). The gene expression and protein concentrations of VEGFR-1 and -2 were increased in CRC tissue compared to normal colorectal tissue, indicating that both receptors are important in CRC. The clinical implications of these findings require further investigation.
血管内皮生长因子A（VEGF-A）是血管生成的关键调节因子，可与受体VEGFR-1和VEGFR-2结合。众所周知，与正常结直肠组织相比，结直肠癌（CRC）组织中VEGF-A水平升高，但关于VEGFR-1和-2受体的表达情况却知之甚少。本研究的目的是对CRC患者肿瘤和正常结直肠组织中VEGFR-1和-2在mRNA和蛋白质水平进行定量分析。从110例行CRC手术切除的患者中收集组织。以β-2-微球蛋白和β-肌动蛋白作为参照基因，通过实时RT-PCR在基因表达水平分析受体状态。使用ELISA技术进行蛋白质分析。与正常结直肠组织相比，CRC组织中VEGFR-1和-2的基因表达及蛋白质浓度显著升高（两种受体P均<10-6）。检测到CRC组织中VEGFR-2的基因表达与疾病分期之间存在关联（P=0.055）。在CRC组织和正常结直肠组织中，VEGFR-1和-2的基因表达之间也发现显著相关性（P<10-4）。与正常结直肠组织相比，CRC组织中VEGFR-1和-2的基因表达及蛋白质浓度升高，表明这两种受体在CRC中均很重要。这些发现的临床意义需要进一步研究。
