Department of Pathology, Inje University Sanggye Paik Hospital, Seoul, Korea.
APMIS. 2011 Jul;119(7):449-59. doi: 10.1111/j.1600-0463.2011.02752.x. Epub 2011 Apr 25.
The purpose of this study was to evaluate the association between the expression of growth factors and the clinicopathological variables of colorectal adenocarcinoma. Immunohistochemistry and fluorescence in situ hybridization (FISH) were used to evaluate the amplification and expression of epidermal growth factor receptor (EGFR), vascular endothelial growth factor (VEGF), VEGF-D, VEGF receptor (VEGFR)-2, VEGFR-3, transforming growth factor (TGF)-β1, and insulin-like growth factor-1 receptor (IGF-1R) in a tissue microarray of 292 colorectal adenocarcinomas. The expression of EGFR, VEGF, VEGF-D, VEGFR-2 and VEGFR-3 was detected in 5.1%, 10.0%, 6.8%, 5.2%, and 57.2%. EGFR expression was associated with angioinvasion (p < 0.05) and lymph node metastasis (p < 0.005). VEGFR-3 expression was higher in the rectum than in the colon (p < 0.05). VEGF expression correlated with VEGF-D (p < 0.05) and VEGFR-3 (p < 0.005) expression, while VEGF-D expression showed no significant association with VEGFR-2 or VEGFR-3. EGFR amplification was present in 10.6% and was not associated with EGFR protein expression. VEGFR-2 and VEGFR-3 expression levels were related to poor patient survival. Stage, perineural invasion, and lymph node metastasis were independent prognostic factors based on a Cox analysis. VEGFR-2 and VEGFR-3 expression are markers of a poor prognosis in patients with surgically resected colorectal adenocarcinoma, whereas EGFR has a minor influence.
本研究旨在评估生长因子的表达与结直肠腺癌临床病理变量之间的关系。采用免疫组织化学和荧光原位杂交（FISH）方法，在 292 例结直肠腺癌组织微阵列中评估表皮生长因子受体（EGFR）、血管内皮生长因子（VEGF）、VEGF-D、VEGF 受体（VEGFR）-2、VEGFR-3、转化生长因子（TGF）-β1 和胰岛素样生长因子-1 受体（IGF-1R）的扩增和表达。EGFR、VEGF、VEGF-D、VEGFR-2 和 VEGFR-3 的表达分别为 5.1%、10.0%、6.8%、5.2%和 57.2%。EGFR 表达与血管浸润（p < 0.05）和淋巴结转移（p < 0.005）有关。VEGFR-3 表达在直肠中高于结肠（p < 0.05）。VEGF 表达与 VEGF-D（p < 0.05）和 VEGFR-3（p < 0.005）表达相关，而 VEGF-D 表达与 VEGFR-2 或 VEGFR-3 无显著相关性。EGFR 扩增率为 10.6%，与 EGFR 蛋白表达无关。VEGFR-2 和 VEGFR-3 的表达水平与患者生存不良相关。基于 Cox 分析，分期、神经周围侵犯和淋巴结转移是独立的预后因素。VEGFR-2 和 VEGFR-3 的表达是手术切除的结直肠腺癌患者预后不良的标志物，而 EGFR 的影响较小。
