Institute of Animal Nutrition, Sichuan Agricultural University, Yaan, Sichuan, People's Republic of China.
J Agric Food Chem. 2011 May 25;59(10):5572-8. doi: 10.1021/jf104129k. Epub 2011 Apr 19.
The purpose of this study was to investigate the effect of chronic cysteamine (CS) supplementation on rat metabolism. Rats received biweekly intragastric administration of either CS-HCl at 250 mg/kg body weight or saline (control) for 4 weeks. The 24 h urine and blood serum samples after the last CS treatment were analyzed by nuclear magnetic resonance (NMR)-based metabolomics, specifically high-resolution (1)H NMR metabolic profiling combined with multivariate statistics. Metabolic effects of CS include decreased serum acetate, trimethylamine-N-oxide, and urine hippurate, together with increased urine dimethylamine, indicating modulation of intestinal microbial metabolism of the rats. A decrease in urine succinate, citric acid, and serum acetoacetate, together with an increase in serum lactate, was also observed, which suggests that CS supplementation results in perturbation of energy metabolism in rats.
本研究旨在探讨慢性半胱胺（CS）补充对大鼠代谢的影响。大鼠每周接受两次胃内给予 CS-HCl（250mg/kg 体重）或生理盐水（对照），共 4 周。最后一次 CS 处理后，通过基于核磁共振（NMR）的代谢组学，特别是高分辨率（1）H NMR 代谢图谱结合多变量统计分析，对 24 小时尿液和血清样本进行分析。CS 的代谢影响包括血清乙酸盐、三甲胺 N-氧化物和尿液马尿酸减少，同时尿液二甲胺增加，提示大鼠肠道微生物代谢的调节。还观察到尿液琥珀酸、柠檬酸和血清乙酰乙酸减少，以及血清乳酸增加，这表明 CS 补充导致大鼠能量代谢紊乱。
