补充绿原酸的大鼠氨基酸和能量代谢的代谢组学分析
Metabolomic analysis of amino acid and energy metabolism in rats supplemented with chlorogenic acid.
作者信息
Ruan Zheng, Yang Yuhui, Zhou Yan, Wen Yanmei, Ding Sheng, Liu Gang, Wu Xin, Liao Peng, Deng Zeyuan, Assaad Houssein, Wu Guoyao, Yin Yulong
机构信息
State Key Laboratory of Food Science and Technology, College of Life Science and Food Engineering, Nanchang University, Rm325, 235 Nanjing Street, Nanchang, 330047, China.
出版信息
Amino Acids. 2014 Sep;46(9):2219-29. doi: 10.1007/s00726-014-1762-7. Epub 2014 Jun 14.
Abstract
This study was conducted to investigate effects of chlorogenic acid (CGA) supplementation on serum and hepatic metabolomes in rats. Rats received daily intragastric administration of either CGA (60 mg/kg body weight) or distilled water (control) for 4 weeks. Growth performance, serum biochemical profiles, and hepatic morphology were measured. Additionally, serum and liver tissue extracts were analyzed for metabolomes by high-resolution (1)H nuclear magnetic resonance-based metabolomics and multivariate statistics. CGA did not affect rat growth performance, serum biochemical profiles, or hepatic morphology. However, supplementation with CGA decreased serum concentrations of lactate, pyruvate, succinate, citrate, β-hydroxybutyrate and acetoacetate, while increasing serum concentrations of glycine and hepatic concentrations of glutathione. These results suggest that CGA supplementation results in perturbation of energy and amino acid metabolism in rats. We suggest that glycine and glutathione in serum may be useful biomarkers for biological properties of CGA on nitrogen metabolism in vivo.
摘要
本研究旨在探讨补充绿原酸（CGA）对大鼠血清和肝脏代谢组的影响。大鼠连续4周每日经胃给予CGA（60毫克/千克体重）或蒸馏水（对照组）。测量生长性能、血清生化指标和肝脏形态。此外，通过基于高分辨率¹H核磁共振的代谢组学和多变量统计分析血清和肝组织提取物的代谢组。CGA不影响大鼠生长性能、血清生化指标或肝脏形态。然而，补充CGA可降低血清中乳酸、丙酮酸、琥珀酸、柠檬酸、β-羟基丁酸和乙酰乙酸的浓度，同时增加血清中甘氨酸的浓度和肝脏中谷胱甘肽的浓度。这些结果表明，补充CGA会导致大鼠能量和氨基酸代谢紊乱。我们认为，血清中的甘氨酸和谷胱甘肽可能是CGA在体内对氮代谢生物学特性的有用生物标志物。
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