Johnson M D, Gray M E, Pepinsky R B, Stahlman M T
Department of Pathology, Vanderbilt University, School of Medicine, Nashville, Tennessee 37232-2561.
J Histochem Cytochem. 1990 Dec;38(12):1841-5. doi: 10.1177/38.12.2147709.
We evaluated the distribution of lipocortin-1 immunoreactivity in 118 immature or mature human hypophyses using the peroxidase-antiperoxidase (PAP) technique with a polyclonal rabbit antiserum against lipocortin-1. Serial sections were evaluated for five pituitary hormones and S-100 protein immunoreactivity to compare their distributions with that of lipocortin-1. Scattered or moderate numbers of cells exhibited lipocortin-1 immunoreactivity in the pars distalis of 89 subjects ranging in age from 27 weeks' gestation to 83 years. Seven immature and seven aged specimens exhibited no immunostaining, while 15 specimens from older individuals exhibited only rare immunostaining. Immunostaining did not appear to co-localize selectively with any specific pituitary hormone, although the distribution of immunoreactivity did overlap that of some corticotrophs and was seen in elongated processes of S-100-containing folliculostellate cells. Lipocortin-1 was also found in epithelial cells lining colloid cysts of the residual pars intermedia in 115 of 118 pituitaries ranging in age from 23 weeks' gestation to 83 years. In many intermediate lobe cysts, lipocortin-1 exhibited a pattern of immunoreactivity that partially overlapped the distribution of S-100 protein immunostaining, although the pattern was not identical. Pre-absorption of anti-lipocortin-1 antiserum with lipocortin-1-coupled Sepharose-4B immunoreactivity resulted in loss of immunoreactivity in both lobes. No lipocortin-1 immunoreactivity was seen in the neurohypophysis.
我们使用针对脂皮质素-1的兔多克隆抗血清，采用过氧化物酶-抗过氧化物酶（PAP）技术，评估了118个未成熟或成熟的人类垂体中脂皮质素-1免疫反应性的分布。对连续切片进行评估，检测五种垂体激素和S-100蛋白的免疫反应性，以比较它们与脂皮质素-1的分布情况。在89名年龄从妊娠27周至83岁的受试者的远侧部中，散在或中等数量的细胞呈现脂皮质素-1免疫反应性。7个未成熟和7个老年标本未显示免疫染色，而15个老年个体的标本仅显示罕见的免疫染色。免疫染色似乎未与任何特定的垂体激素选择性共定位，尽管免疫反应性的分布与一些促肾上腺皮质激素细胞的分布重叠，并且在含S-100的滤泡星状细胞的伸长突起中可见。在118个年龄从妊娠23周至83岁的垂体中，115个垂体的中间部残留胶体囊肿内衬上皮细胞中也发现了脂皮质素-1。在许多中间叶囊肿中，脂皮质素-1呈现出一种免疫反应性模式，部分与S-100蛋白免疫染色的分布重叠，尽管模式并不相同。用与脂皮质素-1偶联的琼脂糖-4B免疫反应性预吸收抗脂皮质素-1抗血清，导致两叶的免疫反应性丧失。在神经垂体中未观察到脂皮质素-1免疫反应性。
