Joanna Reszec, Renata Zalewska, Witold Pepiński, Małgorzata Skawronska, Bernaczyk Piotr, Chyczewski Lech
Departament of Medical Pathomorphology, Medical University of Bialystok, Poland.
Folia Histochem Cytobiol. 2010 Dec;48(4):530-3. doi: 10.2478/v10042-010-0081-4.
Papillomas and squamous cell carcinomas are the most common conjunctival and eyelid lesions. The etiology is still unclear and recently human papillomavirus infection and p53 gene mutation have been taken into consideration. The aim of our study was the evaluation of HPV DNApresence and p53 gene mutation in 45 benign and 38 malignant squamous lesions of the conjunctiva and eyelid. For HPV detection PCR-RFLP and immunohistochemical reaction were used; for p53 gene mutation PCR-SSCP was used. Only 8.8% papillomas, 9.1% squamous cell cancers and 3.7% basal cell cancers (using PCR-RFLP method) and 26.6% papillomas, 7.4% squamous cell cancers and 9.1% basal cell cancers (using immunohisto-chemical reaction) were HPV positive. p53 gene mutation was evaluated in 24.4% papillomas, 54.5% squamous cell cancers and 22.2% basal cell cancers; most commonly in 6 and 7 exon. Human papillomavirus infection, opposite to p53 gene mutation, is not a significant etiological factor of the benign and malignant conjunctival and eyelid lesions development.
乳头状瘤和鳞状细胞癌是结膜和眼睑最常见的病变。其病因仍不清楚，最近人乳头瘤病毒感染和p53基因突变已被纳入考虑范围。我们研究的目的是评估45例结膜和眼睑良性鳞状病变及38例恶性鳞状病变中HPV DNA的存在情况和p53基因突变情况。HPV检测采用PCR-RFLP和免疫组化反应；p53基因突变检测采用PCR-SSCP。仅8.8%的乳头状瘤、9.1%的鳞状细胞癌和3.7%的基底细胞癌（采用PCR-RFLP方法）以及26.6%的乳头状瘤、7.4%的鳞状细胞癌和9.1%的基底细胞癌（采用免疫组化反应）HPV呈阳性。24.4%的乳头状瘤、54.5%的鳞状细胞癌和22.2%的基底细胞癌检测到p53基因突变；最常见于第6和第7外显子。与人乳头瘤病毒感染相反，p53基因突变不是结膜和眼睑良性及恶性病变发展的重要病因。
