Toth J, Karcioglu Z A, Moshfeghi A A, Issa T M, Al-Ma'ani J R, Patel K V
1st Department of Ophthalmology, Semmelweis University, Budapest, Hungary.
Cornea. 2000 Mar;19(2):159-62. doi: 10.1097/00003226-200003000-00007.
The p53 tumor-suppressor gene has been documented to exist in mutated forms in many types of squamous cell carcinoma in the body. Also in conjunctival squamous cell carcinoma, human papillomavirus (HPV) is accepted as an oncogenic factor. The objective of our study was to establish a correlation between p53 overexpression and the presence of HPV infection within tumor tissues from patients with conjunctival squamous cell carcinoma.
Tissue sections obtained from paraffin-embedded conjunctival squamous cell carcinoma specimens from 23 patients were examined with light microscopy, polymerase chain reaction (PCR), and immunohistochemistry.
Seventy-eight percent of tumors were positive for p53, whereas 22% were positive for HPV. The proportion of patients positive for both p53 and HPV was 17%, whereas another 17% of the patients were negative for both p53 and HPV. Therefore no significant disproportion was found in the distribution of patients' HPV status and p53 status (p = 1.00). No significant correlation or linear association was found between the HPV status and p53 status (r = 0.022; p = 0.920).
We could not show any statistical association between abnormal p53 gene-product expression by immunohistochemistry in conjunctival squamous cell carcinomas and HPV infection by PCR detection techniques.
p53肿瘤抑制基因已被证明在体内多种类型的鳞状细胞癌中以突变形式存在。在结膜鳞状细胞癌中，人乳头瘤病毒（HPV）也被认为是一种致癌因素。我们研究的目的是在结膜鳞状细胞癌患者的肿瘤组织中建立p53过表达与HPV感染之间的相关性。
对取自23例患者石蜡包埋的结膜鳞状细胞癌标本的组织切片进行光学显微镜检查、聚合酶链反应（PCR）和免疫组织化学检查。
78%的肿瘤p53呈阳性，而22%的肿瘤HPV呈阳性。p53和HPV均呈阳性的患者比例为17%，而另外17%的患者p53和HPV均呈阴性。因此，在患者的HPV状态和p53状态分布中未发现显著差异（p = 1.00）。在HPV状态和p53状态之间未发现显著相关性或线性关联（r = 0.022；p = 0.920）。
我们未能通过免疫组织化学检测结膜鳞状细胞癌中异常的p53基因产物表达与通过PCR检测技术检测到的HPV感染之间显示出任何统计学关联。
