定量测定胰岛素和表皮生长因子的激素受体复合物在培养成纤维细胞质膜上的侧向扩散系数。
Quantitative determination of the lateral diffusion coefficients of the hormone-receptor complexes of insulin and epidermal growth factor on the plasma membrane of cultured fibroblasts.
作者信息
Schlessinger J, Shechter Y, Cuatrecasas P, Willingham M C, Pastan I
出版信息
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5353-7. doi: 10.1073/pnas.75.11.5353.
Abstract
Fluorescent derivatives of insulin and epidermal growth factor bound to 3T3 mouse fibroblasts are mobile on the cell surface, with similar diffusion coefficients, D approximately (3--5) x 10(-10) cm2/sec at 23 degrees C. Increasing the temperature to 37 degrees C results in rapid receptor immobilization. The immobilization is attributed to aggregation of hormone-receptor complexes, their internalization, or a combination of both processes.
摘要
与3T3小鼠成纤维细胞结合的胰岛素和表皮生长因子的荧光衍生物在细胞表面是可移动的，在23摄氏度时具有相似的扩散系数，D约为(3 - 5)×10(-10)平方厘米/秒。将温度升高到37摄氏度会导致受体迅速固定。这种固定归因于激素-受体复合物的聚集、它们的内化或这两个过程的组合。
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Quantitative determination of the lateral diffusion coefficients of the hormone-receptor complexes of insulin and epidermal growth factor on the plasma membrane of cultured fibroblasts.定量测定胰岛素和表皮生长因子的激素受体复合物在培养成纤维细胞质膜上的侧向扩散系数。
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