单细胞中膜蛋白侧向扩散的测量。
Measurement of membrane protein lateral diffusion in single cells.
作者信息
Edidin M, Zagyansky Y, Lardner T J
出版信息
Science. 1976 Feb 6;191(4226):466-8. doi: 10.1126/science.1246629.
PMID:1246629
Abstract
Fluorescence rapidly returns to spots bleached by a laser beam in the continuous fluorescence of cultured cells labeled on the surface with fluorescein isothiocyanate. The rate of recovery of fluorescence after bleaching can be interpreted as a measure of the lateral diffusion of integral membrane proteins labeled with fluorescein.
摘要
在用异硫氰酸荧光素标记表面的培养细胞的持续荧光中，荧光会迅速回到被激光束漂白的斑点处。漂白后荧光的恢复速率可被解释为对用荧光素标记的整合膜蛋白横向扩散的一种度量。
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