ICORP-SORST-Cell Mechanosensing Project, Japan Science and Technology Agency, Nagoya, Aichi 466-8550, Japan.
Nat Cell Biol. 2011 May;13(5):630-2. doi: 10.1038/ncb2214. Epub 2011 Apr 10.
Ciliates and flagellates temporarily swim backwards on collision by generating a mechanoreceptor potential. Although this potential has been shown to be associated with cilia in Paramecium, the molecular entity of the mechanoreceptor has remained unknown. Here we show that Chlamydomonas cells express TRP11, a member of the TRP (transient receptor potential) subfamily V, in the proximal region of the flagella, and that suppression of TRP11 expression results in loss of the avoiding reaction. The results indicate that Chlamydomonas flagella exhibit mechanosensitivity, despite constant motility, by localizing the mechanoreceptor in the proximal region, where active bending is restricted.
纤毛虫和鞭毛虫在碰撞时会通过产生机械感受器电位暂时向后游动。虽然已经表明这种电位与草履虫的纤毛有关，但机械感受器的分子实体仍然未知。在这里，我们表明，衣藻细胞在鞭毛的近端区域表达了 TRP11，它是 TRP（瞬时受体电位）亚家族 V 的成员，并且抑制 TRP11 的表达会导致回避反应的丧失。结果表明，尽管衣藻鞭毛持续运动，但通过将机械感受器定位于活动弯曲受限的近端区域，它们表现出机械敏感性。
