Cairns E, Gschwender H H, Primke M, Yamakawa M, Traub P, Schweiger H G
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5557-9. doi: 10.1073/pnas.75.11.5557.
Isolated Acetabularia crenulata nuclei were injected with Mengo virus RNA solution and then implanted into anucleate posterior Acetabularia mediterranea cell fragments or fused with Acetabularia ryukyuensis cytoplasts. The injected animal virus RNA was actively translated in the plant cell cytoplasm. Mengo virus proteins were detected and localized in Acetabularia cytoplasts by use of an immunofluorescence method on the first to fifth day after injection.
将分离出的皱波角毛藻细胞核注射到Mengo病毒RNA溶液中,然后植入去核的地中海伞藻后细胞片段中,或与琉球伞藻细胞质体融合。注射的动物病毒RNA在植物细胞质中被积极翻译。在注射后的第一天到第五天,通过免疫荧光法在伞藻细胞质体中检测并定位了Mengo病毒蛋白。