Gurdon J B, Partington G A, De Robertis E M
J Embryol Exp Morphol. 1976 Dec;36(3):541-53.
Nuclei from HeLa and other mammalian cells have been injected into Xenopus oocytes. The synthesis, uptake, and release of RNA and proteins by injected nuclei have been investigated by autoradiography. Injected nuclei which undergo enlargement synthesize RNA continuously for up to 28 days. When oocytes are incubated in [3H]uridine or [3H]guanosine, injected nuclei are labelled nearly as strongly as the nucleoli, but much more strongly than the nucleoplasm of the oocyte's germinal vesicle. Injected nuclei appear to increase their rate of RNA synthesis during incubation in oocytes. This apparent increase in the rate of RNA synthesis is correlated with nuclear enlargement, as well as with the loss of protein from injected nuclei and with their uptake of histone and nonhistone proteins from oocyte cytoplasm. Injected HeLa nuclei lose most of the previously synthesized RNA from their nucleoplasm, but little if any of the RNA from their remaining nucleoli.
已将来自HeLa细胞和其他哺乳动物细胞的细胞核注入非洲爪蟾卵母细胞中。通过放射自显影术研究了注入细胞核的RNA和蛋白质的合成、摄取及释放情况。经历膨大的注入细胞核可连续合成RNA长达28天。当卵母细胞在[3H]尿苷或[3H]鸟苷中孵育时，注入细胞核的标记强度几乎与核仁一样强，但比卵母细胞生发泡的核质要强得多。注入细胞核在卵母细胞孵育期间似乎会提高其RNA合成速率。RNA合成速率的这种明显提高与细胞核膨大、注入细胞核中蛋白质的丢失以及它们从卵母细胞细胞质中摄取组蛋白和非组蛋白相关。注入的HeLa细胞核从其核质中丢失了大部分先前合成的RNA，但来自其剩余核仁的RNA即使有也很少丢失。
