Department of Otolaryngology, Xiangya Hospital, Central South University, Xiangya Road 87, Changsha 410008, Hunan Province, People's Republic of China.
Med Oncol. 2012 Jun;29(2):721-8. doi: 10.1007/s12032-011-9929-5. Epub 2011 Apr 11.
ERp29 is an endoplasmic reticulum (ER) stress-inducible protein. It was found that ERp29 was highly expressed in several cancers and associated with resistance to oxidative and radiation stress, which may serve as a novel target for nasopharyngeal carcinoma (NPC) anticancer approach. In this study, we used immunohistochemistry to detect ERp29 expression in radioresistant and radiosensitive NPC tissues. As a result, ERp29 was up-regulated in radioresistant NPC tissues compared to radiosensitive NPC tissues. We also found that ERp29 knockdown attenuated radioresistance of NPC CNE-1 cells and ERp29 overexpression enhanced radioresistance of NPC CNE-2 cells. When exposed to radiation, ERp29 knockdown CNE-1 cells increased radiation-induced cell apoptosis and ERp29 overexpression CNE-2 cells reduced radiation-induced cell apoptosis. Further, we demonstrated that ERp29 up-regulated the expression of Hsp27. In conclusion, our study supports ERp29 could potentiate resistance to radiation in NPC cells, targeting of ERp29 is a rational strategy in treating radioresistant NPC.
ERp29 是内质网（ER）应激诱导蛋白。研究发现 ERp29 在多种癌症中高表达，并与氧化应激和辐射应激抗性相关，可能成为鼻咽癌（NPC）抗癌方法的新靶点。在这项研究中，我们使用免疫组织化学检测了耐辐射和辐射敏感 NPC 组织中的 ERp29 表达。结果表明，与辐射敏感 NPC 组织相比，耐辐射 NPC 组织中 ERp29 上调。我们还发现，敲低 ERp29 可减弱 NPC CNE-1 细胞的辐射抗性，而过表达 ERp29 可增强 NPC CNE-2 细胞的辐射抗性。当暴露于辐射下时，敲低 ERp29 的 CNE-1 细胞增加了辐射诱导的细胞凋亡，而过表达 ERp29 的 CNE-2 细胞减少了辐射诱导的细胞凋亡。此外，我们证明 ERp29 上调了 Hsp27 的表达。总之，我们的研究支持 ERp29 可增强 NPC 细胞对辐射的抗性，靶向 ERp29 是治疗耐辐射 NPC 的合理策略。
