Department of Otorhinolaryngology-Head & Neck Surgery, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Laryngoscope. 2011 Jun;121(6):1202-7. doi: 10.1002/lary.21794. Epub 2011 Apr 7.
OBJECTIVES/HYPOTHESIS: Our goal was to develop a noninvasive, dynamic imaging method that would further the understanding of head and neck cancer (HNC) tumor growth and local spreading. We developed a novel orthotopic mouse model of HNC with a stable cell line expressing a red fluorescent protein gene to compare a molecular imaging tumor quantification with traditional caliper measurement.
An HNC-tdT stable cell line expressing the tdTomato gene was established, which were injected into the floor of the mouth of nude mice. Tumor growth was constantly monitored using molecular imaging for up to 35 days. The tumors were further evaluated by histologic examination.
Established tumors consistently expressed fluorescent signals that were successfully imaged by molecular imaging during the study. Initial tumor development was detected earlier than caliper measurement would allow. The fluorescent signal quantities of tumors detected by the imaging correlated with the tumor sizes measured by calipers.
This novel animal model represents an orthotopic human HNC model. The tumor can be detected earlier with molecular imaging than by conventional external caliper measurement. Unlike surgical measurement, the tumor can be quantified without disturbing the tumor environment. This model has significant potential for HNC oncologic research.
目的/假设：我们的目标是开发一种非侵入性、动态的成像方法，以进一步了解头颈部癌症（HNC）肿瘤的生长和局部扩散。我们建立了一种新型的表达红色荧光蛋白基因的 HNC 原位小鼠模型，将分子成像肿瘤定量与传统游标卡尺测量进行比较。
建立了一种表达 tdTomato 基因的 HNC-tdT 稳定细胞系，将其注入裸鼠的口腔底部。使用分子成像技术对肿瘤生长进行长达 35 天的连续监测。进一步通过组织学检查对肿瘤进行评估。
成功建立的肿瘤持续表达荧光信号，在研究过程中可通过分子成像成功成像。与游标卡尺测量相比，初始肿瘤的发展更早被检测到。成像检测到的肿瘤荧光信号量与卡尺测量的肿瘤大小相关。
这种新型动物模型代表了一种原位人 HNC 模型。与传统的外部游标卡尺测量相比，分子成像可以更早地检测到肿瘤。与手术测量不同，无需干扰肿瘤环境即可对肿瘤进行定量。该模型对头颈部癌症肿瘤学研究具有重要的潜在应用价值。
