口腔癌原位小鼠模型中淋巴转移的时间特征
Temporal characterization of lymphatic metastasis in an orthotopic mouse model of oral cancer.
作者信息
Szaniszlo Peter, Fennewald Susan M, Qiu Suimin, Kantara Carla, Shilagard Tuya, Vargas Gracie, Resto Vicente A
机构信息
Department of Otolaryngology, UTMB Health Cancer Center, Galveston, Texas; UTMB Health Cancer Center, Galveston, Texas.
出版信息
Head Neck. 2014 Nov;36(11):1638-47. doi: 10.1002/hed.23500. Epub 2014 Jan 27.
BACKGROUND
The overall mortality rate in cases of head and neck squamous cell carcinoma (HNSCC) has not improved over the past 30 years, mostly because of the high treatment failure rate among patients with regionally metastatic disease. To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models.
METHODS
HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2-photon microscopy were used to evaluate tumor progress and spread.
RESULTS
Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis.
CONCLUSION
This orthotopic xenograft model closely mimics several characteristics of human cancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology.
背景
在过去30年中，头颈部鳞状细胞癌（HNSCC）患者的总体死亡率并未改善，这主要是因为区域转移性疾病患者的治疗失败率较高。为了更好地了解导致淋巴转移发生的病理生物学过程，迫切需要相关的动物模型。
方法
将HNSCC细胞系植入无胸腺裸鼠的舌部。采用组织学、免疫组织化学和离体双光子显微镜评估肿瘤进展和扩散情况。
结果
不同HNSCC细胞系的原位异种移植产生了不同的生存模式、肿瘤组织学、疾病进展率和淋巴结转移情况。值得注意的是，所有注射的细胞类型在注射后24小时内均到达淋巴结，但并非所有细胞都发生转移。
结论
这种原位异种移植模型紧密模拟了人类癌症的几个特征，对于专注于淋巴转移发生和病理生物学的转化研究可能具有极高的价值。
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