Women and Children's Research Laboratory, Mineola, NY, USA.
Am J Reprod Immunol. 2011 Oct;66(4):279-85. doi: 10.1111/j.1600-0897.2011.00998.x. Epub 2011 Apr 11.
OBJECTIVE The aim of this study was to determine whether culturing human placental explants under different oxygen tensions will alter expression of pro- and anti-inflammatory mediators. METHODS Placental explant cultures from second-trimester, elective, terminations-of-pregnancy were incubated under 21, 5, or 1% O(2) concentrations for 24 hr in the presence or absence of IL-10. Cytokine concentrations in the conditioned medium were quantified by immunoassay. RESULTS Culture of placental explants under 21, 5, or 1% O(2) concentrations produced hyperoxic (143 ± 1.6 mmHg), normoxic (37 ± 1.6 mmHg), and hypoxic (18.2 ± 1.6 mmHg) pO(2) levels for the maternal-fetal interface in the medium. Oxygen tension had profound effects on basal placental cytokine levels as well as on IL-10-stimulated cytokine production. IL-1β and TNF-α, but not IFN-γ production, was reduced by 21% O(2) . Moreover, 21% O(2) levels increased the anti-inflammatory cytokines IL-10 and IL-13 while 1% O(2) tended to decrease the production of these cytokines. CONCLUSIONS Five percent- O(2) incubation more accurately represents in vivo pO(2) conditions at the maternal-fetal interface. Routine culture of placental explants in room air produces a superphysiologic oxygen tension that tended to increase the production of anti-inflammatory and decrease the production of pro-inflammatory cytokines. In addition, low pO(2) may reduce responsiveness of the placenta to the anti-inflammatory actions of IL-10.
目的 本研究旨在探讨在不同氧张力下培养人胎盘组织是否会改变促炎和抗炎介质的表达。 方法 对来自中期、选择性、妊娠终止的胎盘组织进行培养，在存在或不存在 IL-10 的情况下，在 21%、5%或 1%O2 浓度下孵育 24 小时。通过免疫测定法定量测定条件培养基中的细胞因子浓度。 结果 在 21%、5%或 1%O2 浓度下培养胎盘组织可产生高氧（143±1.6mmHg）、常氧（37±1.6mmHg）和低氧（18.2±1.6mmHg）的母胎界面 pO2 水平。氧张力对基础胎盘细胞因子水平以及 IL-10 刺激的细胞因子产生有深远影响。IL-1β和 TNF-α的产生减少了 21%，而 IFN-γ的产生减少了 21%。此外，21%O2 水平增加了抗炎细胞因子 IL-10 和 IL-13 的产生，而 1%O2 水平则倾向于减少这些细胞因子的产生。 结论 5%O2 孵育更能准确地代表母胎界面的体内 pO2 条件。常规在空气条件下培养胎盘组织会产生超生理氧张力，这可能会增加抗炎细胞因子的产生，减少促炎细胞因子的产生。此外，低 pO2 可能会降低胎盘对 IL-10 抗炎作用的反应性。
