• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

实验性小鼠球孢子菌病中活性氧中间体的作用。

The role of reactive oxygen intermediates in experimental coccidioidomycois in mice.

机构信息

Veterans Affairs San Diego Healthcare System (111F) 3350 La Jolla Village Dr, San Diego, CA 92161, USA.

出版信息

BMC Microbiol. 2011 Apr 11;11:71. doi: 10.1186/1471-2180-11-71.

DOI:10.1186/1471-2180-11-71
PMID:21481258
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3088889/
Abstract

BACKGROUND

Coccidioidomycosis is usually a self-limited infection in immunocompentent people. In immunocompentent human beings second infections due to Coccidioides are very rare, indicating that recovery from infection results in protective immunity. In experimental animals, immunization with several different proteins or attenuated mutants protects against a virulent challenge. To explore what mechanisms are responsible for protective immunity, we investigated the course of Coccidioides infection in the gp91phox knock out mouse that has a defect in the oxidative burst that results in chronic granulomatous disease.

RESULTS

We found that the gp91phox knock out mice were somewhat more resistant to intraperitoneal infection and equally as resistant to low dose intranasal infection, but slightly more susceptible to high dose intranasal infection compared to control mice. The gp91phox knock out mice made a more robust inflammatory response to infection than controls, as measured by histology and production of inflammatory cytokines. The gp91phox knock out mice were as protected by immunization with the recombinant Coccidioides protein Ag2/PRA as the controls were against either intraperitoneal or intranasal infection. Coccidioides immitis arthroconidia and spherules were significantly more resistant to H2O2 treatment in vitro than Aspergillus fumigatus spores.

CONCLUSION

These data suggest that oxidative burst may not be required for protective immunity to coccidioidomycois.

摘要

背景

球孢子菌病在免疫功能正常的人群中通常是一种自限性感染。在免疫功能正常的人群中，由于球孢子菌引起的二次感染非常罕见，这表明感染的恢复会产生保护性免疫。在实验动物中，用几种不同的蛋白质或减毒突变体免疫可预防强毒攻击。为了探讨什么机制负责保护性免疫，我们研究了 gp91phox 敲除小鼠中的球孢子菌感染过程，该小鼠在氧化爆发中存在缺陷，导致慢性肉芽肿病。

结果

我们发现，gp91phox 敲除小鼠对腹腔感染的抵抗力略高，对低剂量鼻腔感染的抵抗力与对照小鼠相当，但对高剂量鼻腔感染的抵抗力略低。与对照小鼠相比，gp91phox 敲除小鼠对感染的炎症反应更为强烈，这可以通过组织学和炎症细胞因子的产生来衡量。gp91phox 敲除小鼠对重组球孢子菌蛋白 Ag2/PRA 的免疫保护作用与对照小鼠对腹腔或鼻腔感染的保护作用相当。与烟曲霉孢子相比，球孢子菌的分生孢子和球形小体在体外对 H2O2 处理的抵抗力明显更强。

结论

这些数据表明，氧化爆发可能不是针对球孢子菌病的保护性免疫所必需的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d39/3088889/f726f695acb5/1471-2180-11-71-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d39/3088889/319c01b2945f/1471-2180-11-71-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d39/3088889/d6fd8627a9b6/1471-2180-11-71-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d39/3088889/62e2958ad6fc/1471-2180-11-71-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d39/3088889/263777f12359/1471-2180-11-71-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d39/3088889/f726f695acb5/1471-2180-11-71-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d39/3088889/319c01b2945f/1471-2180-11-71-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d39/3088889/d6fd8627a9b6/1471-2180-11-71-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d39/3088889/62e2958ad6fc/1471-2180-11-71-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d39/3088889/263777f12359/1471-2180-11-71-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d39/3088889/f726f695acb5/1471-2180-11-71-5.jpg

相似文献

1
The role of reactive oxygen intermediates in experimental coccidioidomycois in mice.实验性小鼠球孢子菌病中活性氧中间体的作用。
BMC Microbiol. 2011 Apr 11;11:71. doi: 10.1186/1471-2180-11-71.
2
Absence of phagocyte NADPH oxidase 2 leads to severe inflammatory response in lungs of mice infected with Coccidioides.吞噬细胞 NADPH 氧化酶 2 的缺失导致感染球孢子菌的小鼠肺部发生严重的炎症反应。
Microb Pathog. 2011 Dec;51(6):432-41. doi: 10.1016/j.micpath.2011.08.003. Epub 2011 Aug 29.
3
A genetically engineered live attenuated vaccine of Coccidioides posadasii protects BALB/c mice against coccidioidomycosis.一种经基因工程改造的波萨达斯球孢子菌减毒活疫苗可保护BALB/c小鼠免受球孢子菌病感染。
Infect Immun. 2009 Aug;77(8):3196-208. doi: 10.1128/IAI.00459-09. Epub 2009 Jun 1.
4
Role of signal sequence in vaccine-induced protection against experimental coccidioidomycosis.信号序列在疫苗诱导的针对实验性球孢子菌病的保护作用中的角色。
Infect Immun. 2002 Jul;70(7):3539-45. doi: 10.1128/IAI.70.7.3539-3545.2002.
5
Protection of mice against Coccidioides immitis intranasal infection by vaccination with recombinant antigen 2/PRA.通过用重组抗原2/PRA接种疫苗保护小鼠免受鼻内感染球孢子菌。
Infect Immun. 2002 Jun;70(6):3287-9. doi: 10.1128/IAI.70.6.3287-3289.2002.
6
Dectin-1 is required for resistance to coccidioidomycosis in mice.Dectin-1 对于小鼠对球虫病的抵抗力是必需的。
mBio. 2013 Feb 5;4(1):e00597-12. doi: 10.1128/mBio.00597-12.
7
Nitric oxide synthase activity has limited influence on the control of Coccidioides infection in mice.一氧化氮合酶活性对控制小鼠球孢子菌感染的影响有限。
Microb Pathog. 2011 Sep;51(3):161-8. doi: 10.1016/j.micpath.2011.03.013. Epub 2011 Apr 14.
8
Dendritic cell-based immunization induces Coccidioides Ag2/PRA-specific immune response.树突状细胞免疫接种诱导球孢子菌 Ag2/PRA 特异性免疫应答。
Vaccine. 2019 Mar 14;37(12):1685-1691. doi: 10.1016/j.vaccine.2019.01.034. Epub 2019 Feb 20.
9
Differences in Host Innate Responses among Coccidioides Isolates in a Murine Model of Pulmonary Coccidioidomycosis.肺球孢子菌病小鼠模型中球孢子菌分离株间宿主固有免疫反应的差异
Eukaryot Cell. 2015 Oct;14(10):1043-53. doi: 10.1128/EC.00122-15. Epub 2015 Aug 14.
10
Coccidioidomycosis: host response and vaccine development.球孢子菌病：宿主反应与疫苗研发
Clin Microbiol Rev. 2004 Oct;17(4):804-39, table of contents. doi: 10.1128/CMR.17.4.804-839.2004.

引用本文的文献

1
Spatiotemporal analysis of lung immune dynamics in lethal infection.致死性感染中肺免疫动力学的时空分析
mBio. 2025 May 14;16(5):e0256224. doi: 10.1128/mbio.02562-24. Epub 2024 Nov 29.
2
The Host Response to Coccidioidomycosis.宿主对球孢子菌病的反应。
J Fungi (Basel). 2024 Feb 25;10(3):173. doi: 10.3390/jof10030173.
3
Immunogenetics associated with severe coccidioidomycosis.与严重球孢子菌病相关的免疫遗传学。

本文引用的文献

1
Coccidioides releases a soluble factor that suppresses nitric oxide production by murine primary macrophages.球腔菌释放一种可溶性因子，抑制鼠原代巨噬细胞一氧化氮的产生。
Microb Pathog. 2011 Feb;50(2):100-8. doi: 10.1016/j.micpath.2010.11.006. Epub 2010 Dec 1.
2
Role of NADPH oxidase in host defense against aspergillosis.NADPH 氧化酶在宿主防御曲霉病中的作用。
Med Mycol. 2011 Apr;49 Suppl 1(Suppl 1):S144-9. doi: 10.3109/13693786.2010.487077. Epub 2010 Jun 21.
3
NADPH oxidase limits innate immune responses in the lungs in mice.
JCI Insight. 2022 Nov 22;7(22):e159491. doi: 10.1172/jci.insight.159491.
4
Early Events in Coccidioidomycosis.球孢子菌病的早期事件。
Clin Microbiol Rev. 2019 Oct 16;33(1). doi: 10.1128/CMR.00112-19. Print 2019 Dec 18.
5
How Environmental Fungi Cause a Range of Clinical Outcomes in Susceptible Hosts.环境真菌如何导致易感宿主产生一系列临床结果。
J Mol Biol. 2019 Jul 26;431(16):2982-3009. doi: 10.1016/j.jmb.2019.05.003. Epub 2019 May 9.
6
Coccidioides immitis and posadasii; A review of their biology, genomics, pathogenesis, and host immunity.粗球腔菌和波萨达斯球腔菌；生物学、基因组学、发病机制和宿主免疫的综述。
Virulence. 2018;9(1):1426-1435. doi: 10.1080/21505594.2018.1509667.
7
The Quest for a Vaccine Against Coccidioidomycosis: A Neglected Disease of the Americas.寻求抗球孢子菌病疫苗：美洲的一种被忽视的疾病
J Fungi (Basel). 2016 Dec 16;2(4):34. doi: 10.3390/jof2040034.
8
Immune Response to Coccidioidomycosis and the Development of a Vaccine.对球孢子菌病的免疫反应及疫苗的研发
Microorganisms. 2017 Mar 16;5(1):13. doi: 10.3390/microorganisms5010013.
9
Card9- and MyD88-Mediated Gamma Interferon and Nitric Oxide Production Is Essential for Resistance to Subcutaneous Coccidioides posadasii Infection.Card9和MyD88介导的γ干扰素和一氧化氮产生对于抵抗皮下播散型波萨达斯球孢子菌感染至关重要。
Infect Immun. 2016 Mar 24;84(4):1166-75. doi: 10.1128/IAI.01066-15. Print 2016 Apr.
10
Gene expression in human fungal pathogen Coccidioides immitis changes as arthroconidia differentiate into spherules and mature.人类真菌病原体粗球孢子菌（Coccidioides immitis）的基因表达在其从分生孢子分化为球体并成熟的过程中发生变化。
BMC Microbiol. 2013 May 28;13:121. doi: 10.1186/1471-2180-13-121.
NADPH 氧化酶限制了小鼠肺部的固有免疫反应。
PLoS One. 2010 Mar 16;5(3):e9631. doi: 10.1371/journal.pone.0009631.
4
Exogenous pentraxin 3 restores antifungal resistance and restrains inflammation in murine chronic granulomatous disease.外源性五聚素3可恢复小鼠慢性肉芽肿病的抗真菌耐药性并抑制炎症。
J Immunol. 2009 Oct 1;183(7):4609-18. doi: 10.4049/jimmunol.0900345. Epub 2009 Sep 4.
5
Chronic granulomatous disease.慢性肉芽肿病。
Clin Rev Allergy Immunol. 2010 Feb;38(1):3-10. doi: 10.1007/s12016-009-8136-z.
6
Nonresolving inflammation in gp91phox-/- mice, a model of human chronic granulomatous disease, has lower adenosine and cyclic adenosine 5'-monophosphate.gp91phox基因敲除小鼠是人类慢性肉芽肿病的模型，其持续性炎症中腺苷和环磷酸腺苷水平较低。
J Immunol. 2009 Mar 1;182(5):3262-9. doi: 10.4049/jimmunol.0801739.
7
Defective tryptophan catabolism underlies inflammation in mouse chronic granulomatous disease.色氨酸分解代谢缺陷是小鼠慢性肉芽肿病炎症的基础。
Nature. 2008 Jan 10;451(7175):211-5. doi: 10.1038/nature06471.
8
Evaluation of two homologous proline-rich proteins of Coccidioides posadasii as candidate vaccines against coccidioidomycosis.对波萨达斯球孢子菌两种富含脯氨酸的同源蛋白作为抗球孢子菌病候选疫苗的评估。
Infect Immun. 2007 Dec;75(12):5777-87. doi: 10.1128/IAI.00807-07. Epub 2007 Sep 17.
9
Human polymorphonuclear leukocytes inhibit Aspergillus fumigatus conidial growth by lactoferrin-mediated iron depletion.人类多形核白细胞通过乳铁蛋白介导的铁消耗抑制烟曲霉分生孢子的生长。
J Immunol. 2007 May 15;178(10):6367-73. doi: 10.4049/jimmunol.178.10.6367.
10
Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein.使用表达为单一蛋白质的两种重组抗原来增强小鼠对波萨达斯球孢子菌致死性呼吸道感染的保护作用。
Vaccine. 2006 Jul 26;24(31-32):5904-11. doi: 10.1016/j.vaccine.2006.04.002. Epub 2006 Apr 18.