Center of Regenerative Medicine in Barcelona, Dr, Aiguader 88, 08003 Barcelona, Spain.
Cell Commun Signal. 2011 Apr 11;9(1):8. doi: 10.1186/1478-811X-9-8.
Embryonic stem cells (ESC) and induced pluripotent stem cells (iPSCs) present a great opportunity to treat and model human disease as a cell replacement therapy. There is a growing pressure to understand better the signal transduction pathways regulating pluripotency and self-renewal of these special cells in order to deliver a safe and reliable cell based therapy in the near future. Many signal transduction pathways converge on two major cell functions associated with self-renewal and pluripotency: control of the cell cycle and apoptosis, although a standard method is lacking across the field. Here we present a detailed protocol to assess the cell cycle and apoptosis of ESC and iPSCs as a single reference point offering an easy to use standard approach across the field.
胚胎干细胞（ESC）和诱导多能干细胞（iPSC）为治疗和模拟人类疾病提供了一个很好的机会，作为细胞替代疗法。为了在不久的将来提供一种安全可靠的基于细胞的治疗方法，人们越来越需要更好地了解调节这些特殊细胞的多能性和自我更新的信号转导途径。许多信号转导途径集中在与自我更新和多能性相关的两个主要细胞功能上：细胞周期和细胞凋亡的控制，尽管该领域缺乏标准方法。在这里，我们提出了一个详细的方案，以评估 ESC 和 iPSC 的细胞周期和细胞凋亡作为一个单一的参考点，为该领域提供了一种易于使用的标准方法。
