Centro de Investigação em Química (CIQ-UP, L4), Departamento de Química, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal.
J Colloid Interface Sci. 2011 Jun 15;358(2):626-34. doi: 10.1016/j.jcis.2011.03.039. Epub 2011 Mar 16.
Thin films composed of oleylamine (OLA) and double-stranded deoxyribonucleic acid (dsDNA from Salmon testes) have been successfully constructed on polycrystalline Au surfaces using the electrostatic adsorption and self-assembly (SA) technique. The formation of the Au/OLA/dsDNA films was followed step-by-step by Quartz Crystal Microbalance with energy dissipation (QCM-D), Atomic Force Microscopy (AFM), and electrochemical techniques such as Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS). The use of these techniques allowed the characterization and the follow up of the successful construction of the OLA/dsDNA composite film. The main advantages of the proposed methodology are the simplicity of the modification procedure, the stability of the dsDNA self-assembled film, and the potential employment of the dsDNA modified gold electrodes to study the interactions of DNA with target molecules.
使用静电吸附和自组装（SA）技术，成功地在多晶金表面构建了由油胺（OLA）和双链脱氧核糖核酸（鲑鱼睾丸中的 dsDNA）组成的薄膜。通过石英晶体微天平（QCM-D）、原子力显微镜（AFM）和电化学技术（如循环伏安法（CV）和电化学阻抗谱（EIS））一步一步地跟踪 Au/OLA/dsDNA 薄膜的形成。这些技术的使用允许对 OLA/dsDNA 复合薄膜的成功构建进行表征和跟踪。所提出方法的主要优点是修饰程序简单、dsDNA 自组装膜的稳定性以及 dsDNA 修饰金电极在研究 DNA 与靶分子相互作用中的潜在应用。
