Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, United Kingdom.
Res Vet Sci. 2012 Apr;92(2):213-8. doi: 10.1016/j.rvsc.2011.03.020. Epub 2011 Apr 9.
Wounds on the limbs of horses are notoriously difficult to heal, with over production of TGFβ1 thought to be responsible for excessive scarring; in contrast, wounds in the oral cavity heal rapidly with minimal scarring. This experiment aimed to determine the effect of TGFβ1 on the production of mRNA and proteins for various extracellular matrix components by two equine fibroblast cell lines isolated from the oral mucosa and distal limb. Fibronectin mRNA was up-regulated by TGFβ1 in the limb but not the oral cells. TGFβ1 increased the ratio of mRNA for collagen types I-III for the oral cells only. mRNA expression for TGFβ receptors-I and -II was significantly lower in limb fibroblasts, and treatment of either cell line with TGFβ1 down-regulated mRNA expression for both receptors. These differences may account for the improved healing seen in oral wounds compared to the excessive scarring seen in limb wounds.
马四肢的创伤很难愈合，这是众所周知的，过多的 TGFβ1 被认为是导致过度瘢痕形成的原因；相比之下，口腔内的伤口愈合迅速，几乎没有瘢痕。本实验旨在确定 TGFβ1 对两种从口腔黏膜和远端肢体分离的马成纤维细胞系产生的各种细胞外基质成分的 mRNA 和蛋白的影响。在肢体细胞中，TGFβ1 上调了纤连蛋白 mRNA 的表达，但在口腔细胞中没有。TGFβ1 仅增加了口腔细胞中 I-III 型胶原 mRNA 的比值。肢体成纤维细胞中 TGFβ 受体-I 和 -II 的 mRNA 表达显著降低，两种细胞系用 TGFβ1 处理后，两种受体的 mRNA 表达均下调。这些差异可能解释了与四肢伤口过度瘢痕形成相比，口腔伤口愈合情况更好的原因。
