Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, Ceredigion SY23 3DA, UK.
Vet J. 2012 Aug;193(2):539-44. doi: 10.1016/j.tvjl.2012.01.010. Epub 2012 Feb 10.
The objective of this study was to determine the effect of platelet derived growth factor BB (PDGF), epidermal growth factor (EGF), transforming growth factor β1 (TGFβ1), insulin like growth factor-1 (IGF-1) and fibroblast growth factor-2 (FGF-2) on the proliferation and migration of equine oral mucosa and leg skin fibroblast cell lines, using an in vitro scratch assay. Fibroblasts from the two sites were firstly grown to confluence and then an area of cells removed (cell void area). Cell migration alone (with the addition of the mitosis inhibitor mitomycin-C to the culture media) and proliferation and migration combined (without mitomycin-C) into the cell void area were observed at 0, 5, 10, 24 and 36 h. The presence of mitomycin-C in the culture media significantly slowed the closure of the cell void area, as mitosis was inhibited. For the oral cells only, TGFβ1 significantly slowed both migration (with mitomycin-C) and proliferation and migration combined (without mitomycin-C). For the limb cells only, both PDGF and FGF-2 significantly increased fibroblast proliferation and migration combined (without mitomycin-C). For both cell types, EGF significantly reduced migration (with mitomycin-C). IGF-1 had no effect on any of the parameters measured. It was concluded that TGFβ1, PDGF and FGF-2 have differential effects on the proliferation and migration of equine oral and limb fibroblasts. These differences in fibroblast responses to growth factors may in part form the basis of the different clinical outcomes for oral and limb wounds.
本研究旨在通过体外划痕试验，确定血小板衍生生长因子 BB（PDGF）、表皮生长因子（EGF）、转化生长因子 β1（TGFβ1）、胰岛素样生长因子-1（IGF-1）和纤维母细胞生长因子-2（FGF-2）对马口腔黏膜和腿部皮肤成纤维细胞系增殖和迁移的影响。首先将来自两个部位的成纤维细胞培养至汇合，然后去除细胞（细胞缺失区）。在 0、5、10、24 和 36 h 时，观察细胞单独迁移（培养基中加入有丝分裂抑制剂丝裂霉素 C）以及增殖和迁移的组合（无丝裂霉素 C）进入细胞缺失区。培养基中丝裂霉素 C 的存在显著减缓了细胞缺失区的闭合，因为有丝分裂受到抑制。对于口腔细胞，仅 TGFβ1 显著减缓了有丝分裂抑制剂存在时的迁移（有丝分裂抑制剂）和增殖和迁移的组合（无丝裂霉素 C）。对于肢体细胞，PDGF 和 FGF-2 均显著增加了无丝裂霉素 C 存在时的成纤维细胞增殖和迁移的组合。对于这两种细胞类型，EGF 显著减少了有丝分裂抑制剂存在时的迁移。IGF-1 对所有测量参数均无影响。结论是，TGFβ1、PDGF 和 FGF-2 对马口腔和肢体成纤维细胞的增殖和迁移有不同的影响。这些成纤维细胞对生长因子反应的差异可能部分构成口腔和肢体伤口临床结果不同的基础。
