Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico.
Cytokine. 2011 Jul;55(1):29-33. doi: 10.1016/j.cyto.2011.03.021. Epub 2011 Apr 9.
Inflammation plays an important role in the pathogenesis of atherosclerosis and acute coronary syndromes (ACS). Interleukin-10 (IL-10) is a potent anti-inflammatory cytokine that mediates the inflammatory process. The objective of the present study was to evaluate the role of IL-10 gene polymorphisms as susceptibility markers for ACS in Mexican patients. IL-10 promoter polymorphisms (positions -1082, -819, and -592) were analyzed by 5' exonuclease TaqMan genotyping assays in 389 ACS patients and 302 healthy controls. ACS patients showed increased frequencies of IL-10-592 C allele and CC genotype when compared to healthy controls (pC=0.0006, OR=1.48 and pC=0.022, OR=1.56, respectively), whereas the frequencies of the A allele and AA genotype were decreased in patients (pC=0.0006, OR=0.68 and pC=0.006, OR=0.57, respectively). When the distribution of IL-10-592 genotypes was analyzed separately in women and men (patients and healthy controls), a different distribution of alleles and genotypes was observed only in the group of men. In this case, increased frequency of C allele (pC=0.004, OR=1.46) and decreased frequencies of A allele (pC=0.004, OR=0.68) and AA genotype (pC=0.023, OR=0.56) were observed in the group of patients when compared to healthy controls. Multiple logistic analyses by gender showed that male individuals with IL-10-592CC+AC genotypes had 3.54-fold increased risk of developing ACS than individuals with AA genotype (p<0.001). The analysis of linkage disequilibrium showed one (ACC) increased haplotype in patients as compared to healthy controls. The results suggest that IL-10 gene polymorphisms could be involved in the risk of developing ACS in the Mexican population.
炎症在动脉粥样硬化和急性冠状动脉综合征（ACS）的发病机制中起着重要作用。白细胞介素-10（IL-10）是一种有效的抗炎细胞因子，可介导炎症过程。本研究的目的是评估 IL-10 基因多态性作为墨西哥患者 ACS 易感性标志物的作用。通过 5'外切酶 TaqMan 基因分型检测分析了 IL-10 启动子多态性（位置-1082、-819 和-592），在 389 例 ACS 患者和 302 例健康对照者中进行了分析。与健康对照组相比，ACS 患者的 IL-10-592C 等位基因和 CC 基因型频率增加（pC=0.0006，OR=1.48 和 pC=0.022，OR=1.56），而 A 等位基因和 AA 基因型频率降低（pC=0.0006，OR=0.68 和 pC=0.006，OR=0.57）。当单独分析女性和男性（患者和健康对照组）中 IL-10-592 基因型的分布时，仅在男性组中观察到等位基因和基因型的分布不同。在这种情况下，与健康对照组相比，患者组中 C 等位基因（pC=0.004，OR=1.46）的频率增加，A 等位基因（pC=0.004，OR=0.68）和 AA 基因型（pC=0.023，OR=0.56）的频率降低。按性别进行的多元逻辑分析显示，与 AA 基因型相比，IL-10-592CC+AC 基因型的男性个体发生 ACS 的风险增加 3.54 倍（p<0.001）。连锁不平衡分析显示，与健康对照组相比，患者中有一个（ACC）增加的单倍型。结果表明，IL-10 基因多态性可能与墨西哥人群发生 ACS 的风险有关。
