Australian Centre for Research on Separation Science (ACROSS), School of Chemistry, University of Tasmania, Hobart, TAS 7001, Australia.
J Chromatogr A. 2011 May 20;1218(20):3007-12. doi: 10.1016/j.chroma.2011.03.046. Epub 2011 Apr 7.
There are many methods available to detect and positively identify either organic or inorganic explosives separately, however no one method has been developed which can detect both types of explosive species simultaneously from a single sample. In this work, a unique coupled-chromatographic system is reported for the simultaneous determination of both organic and inorganic explosive species and is used for pre-blast analysis/identification purposes. This novel approach is based on the combination of reversed-phase high performance liquid chromatography and ion chromatography which allows trace levels of organic and inorganic explosives to be determined simultaneously from a single sample. Using this procedure, a 20 min reversed-phase separation of organic explosives is coupled to a 16 min ion-exchange separation of anions present in inorganic explosives, providing a complete pre-blast analysis/identification system for the separation and detection of a complex mixture containing organic and/or inorganic explosive species. The total analysis time, including sufficient column re-equilibration between runs, was <25 min using the coupled system. By this method, the minimum resolution for the organic separation was 1.16 between nitroglycerin and tetryl and the detection limits ranged from 0.31 mg L(-1) for cyclotetramethylene tetranitramine (HMX) and 1.54 mg L(-1) for pentaerythrite tetranitrate (PETN), while the minimum resolution for the inorganic separation was 0.99 between azide and nitrate, and the detection limits ranged from 7.70 μg L(-1) for fluoride and 159.50 μg L(-1) for benzoate.
有许多方法可用于分别检测和确证有机或无机爆炸物，然而，还没有开发出一种方法能够从单个样品中同时检测这两种类型的爆炸物。在这项工作中，报告了一种独特的偶联色谱系统，用于同时测定有机和无机爆炸物种类，并用于爆炸前分析/鉴定目的。这种新方法基于反相高效液相色谱和离子色谱的组合，允许从单个样品中同时测定痕量水平的有机和无机爆炸物。使用该程序，对有机爆炸物进行 20 分钟的反相分离，然后与无机爆炸物中阴离子的 16 分钟离子交换分离偶联，为分离和检测含有有机和/或无机爆炸物的复杂混合物提供了完整的爆炸前分析/鉴定系统。使用偶联系统，总分析时间（包括运行之间足够的柱重新平衡）<25 分钟。通过该方法，有机分离的最小分辨率为 1.16，甘油三硝酸酯和特屈儿之间，检测限范围为 0.31 毫克/升（HMX）和 1.54 毫克/升（PETN），而无机分离的最小分辨率为 0.99，叠氮化物和硝酸盐之间，检测限范围为 7.70 微克/升（F）和 159.50 微克/升（苯甲酸）。
